State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai, China.
Bioresour Technol. 2010 Nov;101(22):8632-8. doi: 10.1016/j.biortech.2010.06.121.
Transmembrane transports of four kinds of lipophilic organic chemicals (LOCs) on suspending multilamellar liposomes (SML) and Escherichia coli (E. coli) were investigated, where both anthracene and phenanthrene were accorded to the lipid-water partition law and Sudan I and III to the Langmuir isothermal adsorption. Less than half of phenanthrene is transported into E. coli, where more than 60% are located in the cytoplasm. About 60% of anthracene entered the E. coli where only 10% was released into the cytoplasm. The partition coefficients of phenanthrene and anthracene partitioning from the extracellular liquid into membrane are 502 and 1190L/kg but their inverse partition coefficients are only 0.180 and 0.018kg/L. Over 60% of Sudan I and less than 40% of Sudan III accumulated on E. coli where most of them remained on the membrane. The transmembrane impedance effect (TMIE) is proposed for evaluating the cell-transport of polar LOCs.
研究了 4 种疏水性有机化学品（LOCs）在悬浮多层脂质体（SML）和大肠杆菌（E. coli）上的跨膜转运，其中蒽和菲遵循脂质-水分配定律，苏丹 I 和 III 遵循朗缪尔等温吸附。只有不到一半的菲转运进入大肠杆菌，其中 60%以上位于细胞质中。约 60%的蒽进入大肠杆菌，只有 10%释放到细胞质中。从细胞外液进入膜内的菲和蒽的分配系数分别为 502 和 1190L/kg，但它们的反分配系数仅为 0.180 和 0.018kg/L。超过 60%的苏丹 I 和不到 40%的苏丹 III 积累在大肠杆菌上，其中大部分仍保留在膜上。提出了跨膜阻抗效应（TMIE）来评估极性 LOCs 的细胞转运。
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