Graduate school of Energy and Environment, Seoul National University of Science and Technology, 232Gongneung-ro, Nowon-gu, Seoul, 139-743, Republic of Korea.
Nanoscale. 2017 Oct 26;9(41):15998-16004. doi: 10.1039/c7nr05545e.
Glucose oxidase (GOx) and polyacrylic acid (PAA) based water swellable non-toxic enzyme-polymer conjugate (PAA-GOx) was immobilized on a substrate consisting of graphene oxide (GO) and polyethyleneimine (PEI) (GO-PEI) and the electrochemical performances of the new catalyst were investigated. According to the measurements, although the amount of GOx immobilized on PAA-GOx was lower than that on glutaraldehyde (GA)-GOx, which is a conventionally used conjugate, its catalytic activity was 9.6 times higher than that of GA-GOx. The superior catalytic activity (102.0 μA cm, 20 mM of glucose) and glucose sensitivity (6.9 μA cm mM) were due to its high swellability in water. Due to this, the PAA-GOx absorbs a large amount of aqueous glucose molecules and rapidly transfers them to the active site of GOx. Desirable hydrogen peroxide and glucose oxidation reactions are accordingly promoted. In addition, since PAA has abundant free carboxylic acid groups, the PAA-GOx forms covalent bonds with the GO-PEI to curtail the leaching-out of GOx molecules.
基于葡萄糖氧化酶 (GOx) 和聚丙烯酸 (PAA) 的水膨胀无毒酶-聚合物缀合物 (PAA-GOx) 被固定在由氧化石墨烯 (GO) 和聚乙烯亚胺 (PEI) 组成的基底上 (GO-PEI)，并研究了新催化剂的电化学性能。根据测量结果，尽管固定在 PAA-GOx 上的 GOx 量低于传统使用的缀合物戊二醛 (GA)-GOx，但它的催化活性是 GA-GOx 的 9.6 倍。其优越的催化活性（102.0 μA cm，20 mM 葡萄糖）和葡萄糖敏感性（6.9 μA cm mM）归因于其在水中的高溶胀性。因此，PAA-GOx 吸收大量的水溶液葡萄糖分子，并迅速将其转移到 GOx 的活性位点。相应地，促进了理想的过氧化氢和葡萄糖氧化反应。此外，由于 PAA 具有丰富的游离羧酸基团，PAA-GOx 与 GO-PEI 形成共价键，以限制 GOx 分子的浸出。
