Kobyliak Nazarii, Falalyeyeva Tetyana, Beregova Tetyana, Spivak Mykola
Bogomolets National Medical University.
Endokrynol Pol. 2017;68(6):659-667. doi: 10.5603/EP.a2017.0055. Epub 2017 Oct 12.
a comparative animal study of the efficacy of intermittent short-course administration of lyophilised single-, three-, and live multistrain probiotic on obesity.
We included 70 rats divided into seven groups (n = 10 in each). Rats of group I were intact. Newborn rats of groups II-VII were injected with monosodium glutamate (MSG) (4 mg/g). Rats of group II (MSG-obesity group) were untreated. The group III-V received lyophilised monoprobiotics B. animalis VKL, B. animalis VKB, and L. casei IMVB-7280, respectively. Group VI received the mix of these three probiotic strains. Group VII was treated with multiprobiotic "Symbiter", which contains 14 live probiotic strains (Lactobacillus, Bifi-dobacterium, Propionibacterium, Acetobacter genera).
Neonatal treatment with MSG caused stunted growth, which is why, despite the lack of weight gain dynamics and absence of significant food consumption rate and body weight changes at day 120, we noted the development of obesity in all MSG-obesity rats and in up to 20-70% after probiotic administration. Supplementation of probiotic composition, with preference to live strains, led to a significantly lower prevalence of obesity, and reduction of VAT weight and serum lipid levels as compared to single-strain probiotic. In our comparative single-strain analysis a trend towards more pronounced hypolipidaemic effect and VAT weight reduction was observed for lyophilised L. casei IMVB-7280 as compared to B. animalis VKL and VKB strains.
Multistrain formed mutualistic interactions in mixtures and therefore able to share with different metabolites, affect differ-ent receptors and produced various of biologically active compounds which synergistic overall effect greater than the sum of the single effects.
一项关于冻干单菌株、三菌株和活多菌株益生菌间歇性短疗程给药对肥胖疗效的比较动物研究。
我们纳入了70只大鼠，分为七组（每组n = 10）。第一组大鼠未做处理。第二至七组的新生大鼠注射了味精（MSG）（4毫克/克）。第二组（MSG肥胖组）未接受治疗。第三至五组大鼠分别接受冻干单菌株益生菌动物双歧杆菌VKL、动物双歧杆菌VKB和干酪乳杆菌IMVB - 7280。第六组接受这三种益生菌菌株的混合物。第七组用含有14种活益生菌菌株（乳酸杆菌属、双歧杆菌属、丙酸杆菌属、醋酸杆菌属）的多菌株益生菌“Symbiter”进行治疗。
新生儿期用味精治疗导致生长发育迟缓，这就是为什么尽管在第120天时体重没有增加动态，食物消耗率和体重也没有显著变化，但我们注意到所有MSG肥胖大鼠以及在给予益生菌后高达20 - 70%的大鼠出现了肥胖。与单菌株益生菌相比，补充益生菌组合物，尤其是活菌株，导致肥胖患病率显著降低，内脏脂肪组织（VAT）重量和血脂水平降低。在我们的比较单菌株分析中，与动物双歧杆菌VKL和VKB菌株相比，观察到冻干干酪乳杆菌IMVB - 7280有更明显的降血脂作用和VAT重量降低的趋势。
多菌株在混合物中形成互利共生相互作用，因此能够共享不同代谢产物，影响不同受体并产生各种生物活性化合物，其协同总体效应大于单一效应之和。
