Kobyliak Nazarii, Falalyeyeva Tetyana, Tsyryuk Olena, Eslami Majid, Kyriienko Dmytro, Beregova Tetyana, Ostapchenko Liudmila
Department Endocrinology, Bogomolets National Medical University, T. Shevchenko boulevard, 13, Kyiv, 01601 Ukraine.
Taras Shevchenko National University of Kyiv, Volodymyrska Str., 64/13, Kyiv, 01601 Ukraine.
J Diabetes Metab Disord. 2020 Feb 16;19(1):289-296. doi: 10.1007/s40200-020-00506-3. eCollection 2020 Jun.
сomparative animal study of effectiveness of intermittent administration of lyophilized single-, three- and alive multistrain probiotic in short courses on insulin resistance (IR) in rats with experimental obesity.
70 rats were divided into 7 groups ( = 10 in each). Rats of group I were left intact. Newborn rats in groups II-VII were administered monosodium glutamate (MSG) (4 mg/g) by injection. Rats in group II (MSG-obesity group) were left untreated. The rats in groups III-V received lyophilized mono-probiotics , , respectively. The rats in group VI received all three of these probiotic strains mixed together. Group VII was treated with multi-probiotic "Symbiter", containing 14 different live probiotic strains (, , , genera).
Treatment of newborn rats with MSG lead to the development of obesity in all MSG-obesity rats and up to 20-70% after probiotic administration. Additions to probiotic composition, with preference to alive strains (group VII), led to significantly lower rates of obesity, decrease in HOMA-IR ( < 0.001), proinflammatory cytokines levels - IL-1β ( = 0.003), IL-12Bp40 (p < 0.001) and elevation of adiponectin (p = 0.003), TGF-β ( = 0.010) in comparison with MSG-obesity group. Analysis of results in groups treated with single-strain probiotics (groups III-V) shows significant decrease in HOMA-IR, but changes were less pronounced as compared to mixture groups and did not achieve intact rats level. Other metabolic parameters were not affected significantly by single strains.
Our findings provide major clues for how to design and use probiotics with more efficient compositions in obesity and IR management and may bring new insights into how host-microbe interactions contribute to such protective effects.
对冻干的单菌株、三菌株和活性多菌株益生菌短疗程间歇性给药对实验性肥胖大鼠胰岛素抵抗（IR）有效性的比较动物研究。
70只大鼠分为7组（每组10只）。I组大鼠不做处理。II - VII组新生大鼠通过注射给予味精（MSG）（4mg/g）。II组（MSG - 肥胖组）不做处理。III - V组大鼠分别接受冻干的单菌株益生菌、、。VI组大鼠接受这三种益生菌菌株混合在一起的处理。VII组大鼠用含有14种不同活性益生菌菌株（、、、属）的多菌株益生菌“Symbiter”治疗。
用MSG处理新生大鼠导致所有MSG - 肥胖大鼠肥胖，益生菌给药后肥胖率高达20 - 70%。益生菌组合物中添加，尤其是活性菌株（VII组），导致肥胖率显著降低，HOMA - IR降低（<0.001），促炎细胞因子水平 - IL - 1β（=0.003）、IL - 12Bp40（p<0.001）降低，脂联素（p = 0.003）、TGF - β（=0.010）升高，与MSG - 肥胖组相比。对单菌株益生菌处理组（III - V组）结果的分析显示HOMA - IR显著降低，但与混合组相比变化不明显，未达到未处理大鼠水平。单菌株对其他代谢参数无显著影响。
我们的研究结果为如何设计和使用具有更有效组合物的益生菌来管理肥胖和IR提供了重要线索，并可能为宿主 - 微生物相互作用如何产生这种保护作用带来新的见解。
