Zhao Chengcheng, Ying Zheming, Tao Xiaojun, Jiang Mingyue, Ying Xixiang, Yang Guanlin
a School of Pharmacy , Liaoning University of Traditional Chinese Medicine , Dalian , China.
b School of the First Clinic , Liaoning University of Traditional Chinese Medicine , Shenyang , Liaoning , China.
Nat Prod Res. 2018 Jul;32(13):1548-1553. doi: 10.1080/14786419.2017.1385022. Epub 2017 Oct 12.
A new lactam alkaloid named oleraciamide D (1), indentified as (5R)-4-(3-methoxy-4-hydroxyphenyl)-5-(4-hydroxyphenyl)-5,6-dihydropyridin-2(1H)-one, together with five known compounds, indole-3-aldehyde (2), portulacatone (3), N-trans-feruloyloctopamine (4), N-trans-feruloyl-3'-O-methyldopamine (5) and N-trans-feruloyltyramine (6) were isolated from Potulaca oleracea L. Among them, indole-3-aldehyde (2) was isolated from the medicine for the first time. The structure of the new alkaloid was elucidated via UHPLC-ESI-Q-TOF/MS, 1D NMR and 2D NMR. The five known compounds were established by comparing the H-NMR and C NMR with the reported literature. Oleraciamide D (1) showed cytotoxicity against SH-SY5Y cells when concentration at 50 uM by CCK-8 method.
一种名为马齿苋酰胺D（1）的新型内酰胺生物碱，被鉴定为（5R）-4-（3-甲氧基-4-羟基苯基）-5-（4-羟基苯基）-5,6-二氢吡啶-2（1H）-酮，与五种已知化合物，吲哚-3-甲醛（2）、马齿苋酮（3）、N-反式阿魏酰章鱼胺（4）、N-反式阿魏酰-3'-O-甲基多巴胺（5）和N-反式阿魏酰酪胺（6）一起从马齿苋中分离得到。其中，吲哚-3-甲醛（2）首次从该药物中分离得到。通过超高效液相色谱-电喷雾电离-四极杆-飞行时间质谱（UHPLC-ESI-Q-TOF/MS）、一维核磁共振（1D NMR）和二维核磁共振（2D NMR）对新生物碱的结构进行了阐明。通过将氢核磁共振（H-NMR）和碳核磁共振（C NMR）与已报道的文献进行比较，确定了这五种已知化合物。采用CCK-8法检测，当马齿苋酰胺D（1）浓度为50 μM时，对SH-SY5Y细胞显示出细胞毒性。
