School of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian, PR China.
Department of Pharmacy, First Affiliated Hospital of Dalian Medical University, Dalian, PR China.
Fitoterapia. 2019 Jul;136:104166. doi: 10.1016/j.fitote.2019.05.005. Epub 2019 May 7.
Two new indole alkaloids, identified as (E)-1-(5,6-dihydroxy-1H-indol-1-yl)-3-(4-hydroxyphenyl)prop-2-en-1-one, named oleraindole A (1), and (E)-1-(5,6-dihydroxy-1H-indol-1-yl)-3-(4-hydroxy-3-methoxyphenyl)prop-2-en-1-one, named oleraindole B (2), together with five known indole alkaloids, (-)-neoechinulin A (3), neoechinulin D (4), isoechinulin A (5), MT-6 (6) and echinulin (7) were isolated from the aqueous extract of Portulaca oleracea L. for the first time, using various chromatographic techniques. The structures of seven alkaloids were elucidated by the 1D and 2D NMR and HR-ESI-TOF-MS spectroscopic methods. Oleraindole A (1) and oleraindole B (2) exhibited a relatively high anticholinesterase activity with IC values of 55.12 ± 0.20 μΜ and 46.76 ± 0.08 μM, and antioxidant activities with the IC values of 16.20 ± 0.11 μM and 13.88 ± 0.06 μM among seven alkaloids.
两种新的吲哚生物碱，鉴定为（E）-1-（5,6-二羟基-1H-吲哚-1-基）-3-（4-羟基苯基）-2-丙烯-1-酮，命名为奥利因吲哚 A（1）和（E）-1-（5,6-二羟基-1H-吲哚-1-基）-3-（4-羟基-3-甲氧基苯基）-2-丙烯-1-酮，命名为奥利因吲哚 B（2），与五种已知的吲哚生物碱，（-）-新鱼腥素 A（3）、新鱼腥素 D（4）、异鱼腥素 A（5）、MT-6（6）和海葵素（7）一起，从马齿苋（Portulaca oleracea L.）的水提取物中首次通过各种色谱技术分离得到。通过 1D 和 2D NMR 和 HR-ESI-TOF-MS 光谱方法阐明了七种生物碱的结构。奥利因吲哚 A（1）和奥利因吲哚 B（2）表现出相对较高的乙酰胆碱酯酶抑制活性，IC 值分别为 55.12 ± 0.20 μΜ 和 46.76 ± 0.08 μΜ，以及抗氧化活性，在七种生物碱中，IC 值分别为 16.20 ± 0.11 μM 和 13.88 ± 0.06 μM。
