Da O, Yerbanga R S, Traore/Coulibaly M, Koama B K, Kabre Z, Tamboura S, Dakuyo Z P, Sekhoacha M P, Matsabisa M G, Nikiema J B, Ouedraogo J B, Ouedraogo G A
Direction Regionale de l'Institut de Recherche en Sciences de la Sante (IRSS-DRO/CNRST), BP 545 Bobo-Dioulasso, Burkina Faso.
Centre Muraz, BP390, Bobo-Dioulasso, Burkina Faso.
Pak J Biol Sci. 2016;19(4):171-178. doi: 10.3923/pjbs.2016.171.178.
Cassia alata L. (Fabaceae), one of the three plants contained in Saye, a polyherbal antimalarial remedy was assessed for its antimalarial potential and safety in mice.
Organic extracts were prepared from the leaves and tested on the D 10 chloroquine-sensitive strain of Plasmodium falciparum using the parasite lactate dehydrogenase assay. The 4 days suppressive test using Plasmodium berghei in mice was used to evaluate the in vivo antiplasmodial activity of the extracts. Animals were treated by oral route, once a day with 50, 100, 250 and 400 mg kg -1 b.wt., of the extracts. The acute toxicity of the extracts was assessed in mice according to Thompson and Weil method. The lethal effects of the extracts on animal's body weight, tissues, biochemical and haematological parameters were determined at 823.5, 1235.5, 1853 and 2779.5 mg kg -1 b.wt., respectively.
The dichloromethane/methane (1:1, v/v) extract of Cassia alata was the most active against Plasmodium falciparum. The mean percent suppression of parasitemia in mice was equal to 22.5, 41.8 and 45.2% at 50, 250 and 400 mg kg -1 b.wt., respectively. No death and no clinically significant changes were recorded in mice. The maximum non-lethal dose was more than 16875 mg kg -1 in animals. No significant changes were observed in body weight, tissues morphology, biochemical and hematological parameters at doses above or equal to 2779.5 mg kg -1 b.wt.
The dichloromethane/methanol leaf extract of Cassia alata had a good to moderate in vitro and in vivo antiplasmodial activity and was found to have low toxicity at high doses in tested animals.
对复方抗疟药物Saye中含有的三种植物之一的翅荚决明（豆科）在小鼠体内的抗疟潜力和安全性进行评估。
从叶片中制备有机提取物，并使用寄生虫乳酸脱氢酶测定法对恶性疟原虫的D10氯喹敏感株进行测试。采用伯氏疟原虫对小鼠进行4天抑制试验，以评估提取物的体内抗疟活性。动物通过口服途径给药，每天一次，提取物剂量分别为50、100、250和400mg/kg体重。根据汤普森和韦尔方法评估提取物在小鼠中的急性毒性。分别在823.5、1235.5、1853和2779.5mg/kg体重下测定提取物对动物体重、组织、生化和血液学参数的致死作用。
翅荚决明的二氯甲烷/甲醇（1:1，v/v）提取物对恶性疟原虫活性最强。在50、250和400mg/kg体重时，小鼠体内疟原虫血症的平均抑制百分比分别为22.5%、41.8%和45.2%。小鼠未出现死亡，也未记录到临床上的显著变化。动物的最大非致死剂量超过16875mg/kg。在剂量高于或等于2779.5mg/kg体重时，未观察到体重、组织形态、生化和血液学参数有显著变化。
翅荚决明的二氯甲烷/甲醇叶提取物在体外和体内具有良好至中等的抗疟活性，并且在测试动物中高剂量时毒性较低。
