Sangnoi Yutthapong, Chankaew Sunipa, O-Thong Sompong
Department of Aquatic Science, Faculty of Natural Resources, Prince of Songkla University, Hat Yai, Songkhla, Thailan.
Department of Biology, Faculty of Science, Thaksin University, Phattalung, Thailand.
Pak J Biol Sci. 2017;20(1):52-58. doi: 10.3923/pjbs.2017.52.58.
Toxic nitrogen compounds are one cause decreasing of shrimp production and water pollution. Indigenous Halomonas spp., isolated from Pacific white shrimp farm are benefitted for saline ammonium waste water treatment. This study aimed to isolate the heterotrophic-halophilic Halomonas spp. and investigate their ammonium removal efficiency.
Halomonas spp., were isolated by culturing of samples collected from shrimp farm into modified Pep-Beef-AOM medium. Ammonium converting ability was tested and monitored by nitrite reagent. Ammonium removal efficiency was measured by the standard colorimetric method. Identification and classification of Halomonas spp., were studied by morphological, physiological and biochemical characteristics as well as molecular information.
There were 5 strains of heterotrophic-halophilic nitrifying bacteria including SKNB2, SKNB4, SKNB17, SKNB20 and SKNB22 were isolated. The identification result based on 16S rRNA sequence analysis indicated that all 5 strains were Halomonas spp., with sequence similarity values of 91-99 %. Ammonium removal efficiency of all strains showed a range of 23-71%. The production of nitrite was low detected of 0.01-0.15 mg-N L-1, while the amount of nitrate was almost undetectable.
This might suggest that the indigenous Halomonas spp., as nitrifying bacteria involved biological nitrification process for decreasing and transforming of ammonia. Due to being heterotrophic, halophilic and ammonium removing bacteria, these Halomonas spp., could be developed for use in treatment of saline ammonium waste water.
有毒含氮化合物是导致虾产量下降和水污染的原因之一。从太平洋白虾养殖场分离出的本土嗜盐单胞菌属细菌，有利于处理含盐铵废水。本研究旨在分离异养嗜盐嗜盐单胞菌属细菌，并研究它们的铵去除效率。
通过将从虾养殖场采集的样品接种到改良的Pep-牛肉-AOM培养基中，分离嗜盐单胞菌属细菌。用亚硝酸盐试剂测试和监测铵转化能力。用标准比色法测量铵去除效率。通过形态学、生理学和生化特征以及分子信息研究嗜盐单胞菌属细菌的鉴定和分类。
分离出5株异养嗜盐硝化细菌，分别为SKNB2、SKNB4、SKNB17、SKNB20和SKNB22。基于16S rRNA序列分析的鉴定结果表明，所有5株菌株均为嗜盐单胞菌属细菌，序列相似性值为91%-99%。所有菌株的铵去除效率在23%-71%之间。亚硝酸盐的产量较低，检测到的含量为0.01-0.15 mg-N L-1，而硝酸盐的含量几乎检测不到。
这可能表明，作为硝化细菌的本土嗜盐单胞菌属细菌参与了生物硝化过程，以减少和转化氨。由于这些嗜盐单胞菌属细菌是异养、嗜盐和铵去除细菌，因此可开发用于处理含盐铵废水。
