视网膜色素上皮中黑素小体的波长依赖性光学特性及其随黑色素漂白的变化：一项数值研究。
Wavelength-dependent optical properties of melanosomes in retinal pigmented epithelium and their changes with melanin bleaching: a numerical study.
作者信息
Song Weiye, Zhang Lei, Ness Steve, Yi Ji
机构信息
Department of Medicine, Boston University School of Medicine, Boston, MA 02118, USA.
Department of Ophthalmology, Boston University School of Medicine, Boston, MA 02118, USA.
出版信息
Biomed Opt Express. 2017 Aug 7;8(9):3966-3980. doi: 10.1364/BOE.8.003966. eCollection 2017 Sep 1.
Abstract
In this paper, we present the first numerical study on full metrics of wavelength-dependent optical properties of melanosomes in retinal pigmented epithelial (RPE) cells. T-matrix method was used to simulate the spheroidal shapes of mature melanosomes, and the complex refractive index was calculated by a subtractive Kramers-Kronig relation for melanin. The validity of the method was first confirmed by Mie theory, and corroborated by a comparison between visible light and near infrared (NIR) optical coherence tomography (OCT) on human retinal imaging. We also studied the changes of melanosome optical properties due to melanin bleaching by numerically reducing the absorption of melanin. This study implies a unique approach to detect melanin changes specifically in RPE by a spectroscopic contrast of optical coherence tomography.
摘要
在本文中，我们首次对视网膜色素上皮（RPE）细胞中黑素小体的波长依赖性光学特性的完整度量进行了数值研究。采用T矩阵方法模拟成熟黑素小体的球形形状，并通过对黑色素的减法克喇末-克朗尼格关系计算复折射率。该方法的有效性首先通过米氏理论得到证实，并通过对人体视网膜成像的可见光和近红外（NIR）光学相干断层扫描（OCT）之间的比较得到进一步验证。我们还通过数值降低黑色素的吸收来研究由于黑色素漂白导致的黑素小体光学特性的变化。这项研究意味着一种独特的方法，即通过光学相干断层扫描的光谱对比度来特异性检测RPE中的黑色素变化。
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