Laboratório de Virologia, Departamento de Microbiologia, Imunologia e Parasitologia, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
Laboratório de Virologia, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
Transbound Emerg Dis. 2018 Feb;65(1):5-9. doi: 10.1111/tbed.12735. Epub 2017 Oct 12.
Two full-genome sequences of porcine circovirus type 3 (PCV3) are reported. The genomes were recovered from pooled serum samples from sows who had just delivered litters with variable numbers of stillbirths. The two circular genomes (PCV3-BR/RS/6 and PCV3-BR/RS/8) are 2,000 nucleotides long and contain two open reading frames (ORFs) oriented in opposite directions that encode the putative capsid (Cap) and replicase (Rep) proteins. The intergenic region contains a stem-loop motif, as reported for other circoviruses. Rolling circle replication motifs and putative helicase domains were identified in the Rep coding region. The degree of overall nucleotide similarity between the genomes reported here and those available at GenBank was higher than 97%. No PCV3 sequence was detected in pooled serum samples from sows which had no stillbirths on the same farms. However, further studies are necessary to confirm the association between PCV3 and the occurrence of stillbirths.
现报道两株猪圆环病毒 3 型（PCV3）的全基因组序列。这两个环状基因组（PCV3-BR/RS/6 和 PCV3-BR/RS/8）均长 2000 个核苷酸，包含两个以相反方向编码假定衣壳（Cap）和复制酶（Rep）蛋白的开放阅读框（ORF）。基因间区含有一个发夹环模体，与其他圆环病毒的报道一致。在 Rep 编码区中鉴定出滚环复制基序和假定解旋酶结构域。与 GenBank 中可用序列相比，这里报道的基因组之间的总体核苷酸相似性高于 97%。在同一农场没有死产的母猪的混合血清样本中未检测到 PCV3 序列。然而，需要进一步的研究来确认 PCV3 与死产发生之间的关联。
