Mehrabadi Mohammad Hosein Fallah, Bahonar Alireza, Mirzaei Kamran, Molouki Aidin, Ghalyanchilangeroudi Arash, Ghafouri Seyed Ali, Tehrani Farshad, Lim Swee Hua Erin
Department of Poultry Diseases, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran.
Department of Food Hygiene & Quality Control, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
Trop Anim Health Prod. 2018 Mar;50(3):677-682. doi: 10.1007/s11250-017-1438-x. Epub 2017 Oct 13.
Avian influenza virus (AIV) H9N2 subtype is endemic in Iran and causes substantial economic loss to the growing poultry industry within the country. In this study, a cross-sectional analysis was carried out to determine the sero-prevalence of H9N2 in several commercial farms between the years 2014 and 2015. The comparison of the mean of serum titers and the ratio of sero-positive birds between all units were analyzed using one-way ANOVA test. In 2014, a total of 77 farms (58 turkey farms, 14 quail farms, and 5 partridge farms) and 894 birds (682 turkeys, 154 quails, and 58 partridges) were sampled while in 2015, a total of 69 farms (54 turkey farms, 8 quail farms, and 7 partridge farms) and 856 birds (675 turkeys, 105 quails, and 76 partridges) were sampled. Of that, 52 of 77 sampled farms (67.5%) and 437 of 894 samples (48.9%) were positive for H9N2 in 2014 while. Forty-one of 69 farms (59.4%) and 307 of 856 sera (35.9%) were positive in 2015. Furthermore, the mean titer of partridge farms was significantly lower than that of turkey farms (p < 0.01) and the mean percentage of sero-positive turkey farms was significantly higher than partridge farms (p < 0.01) in 2014. In 2015, no significant difference was observed between the mean sera titer amongst farms and percentage of sero-positive birds (p > 0.05). Our results indicated that H9N2 is circulating in these farms. Since many more such farms are being established for operations, in addition to the threat of emergence and continuous reemergence of the disease in these farms, enhanced veterinary biosecurity measures on farms are required for mitigation.
H9N2亚型禽流感病毒在伊朗呈地方流行性，给该国不断发展的家禽业造成了巨大经济损失。在本研究中，开展了一项横断面分析，以确定2014年至2015年间若干商业养殖场中H9N2的血清流行率。使用单因素方差分析对所有养殖场之间的血清滴度均值和血清阳性禽类比例进行比较分析。2014年，共对77个养殖场（58个火鸡养殖场、14个鹌鹑养殖场和5个鹧鸪养殖场）和894只禽类（682只火鸡、154只鹌鹑和58只鹧鸪）进行了采样，而在2015年，共对69个养殖场（54个火鸡养殖场、8个鹌鹑养殖场和7个鹧鸪养殖场）和856只禽类（675只火鸡、105只鹌鹑和76只鹧鸪）进行了采样。其中，2014年77个采样养殖场中有52个（67.5%）以及894个样本中有437个（48.9%）H9N2呈阳性，而2015年69个养殖场中有41个（59.4%）以及856份血清中有307份（35.9%）呈阳性。此外，2014年鹧鸪养殖场的平均滴度显著低于火鸡养殖场（p < 0.01），血清阳性火鸡养殖场的平均百分比显著高于鹧鸪养殖场（p < 0.01）。2015年，各养殖场之间的血清平均滴度和血清阳性禽类百分比未观察到显著差异（p > 0.05）。我们的结果表明H9N2在这些养殖场中传播。由于正在建立更多这样的养殖场用于经营，除了这些养殖场中疾病出现和持续再次出现的威胁外，还需要加强养殖场的兽医生物安全措施以减轻影响。
