Dracopoli N C, Stanger B Z, Ito C Y, Call K M, Lincoln S E, Lander E S, Housman D E
Center for Cancer Research, Massachusetts Institute of Technology, Cambridge 02139.
Am J Hum Genet. 1988 Oct;43(4):462-70.
A genetic linkage map of 27 loci on the short arm of human chromosome 1 has been developed by analysis of the 40 families in the Centre d'Etude du Polymorphisme Humain (CEPH) reference panel. Probes that recognize 14 novel RFLPs at loci designated D1S9-D1S22 were isolated from a flow-sorted chromosome 1 library. A linkage map of chromosome 1p was constructed from the genotypic data at these 14 loci, RFLPs at eight cloned genes (PND, ALPL, FUCA1, SRC2, MYCL, GLUT, TSHB, and NGFB), two previously identified RFLPs (D1S2 and D1S57), two blood group antigens (RH and FY), and the isozyme PGM1. All 27 loci form a continuous linkage group, from FY to PND, of 102 cM in males and 230 cM in females. This map provides a basis for highly informative multipoint mapping studies for most of the short arm of chromosome 1.
通过对人类多态性研究中心（CEPH）参考家系中的40个家庭进行分析，绘制了人类1号染色体短臂上27个基因座的遗传连锁图谱。从一个经流式细胞分选的1号染色体文库中分离出了在命名为D1S9 - D1S22的基因座上识别14个新的限制性片段长度多态性（RFLP）的探针。利用这14个基因座的基因型数据、8个克隆基因（PND、ALPL、FUCA1、SRC2、MYCL、GLUT、TSHB和NGFB）上的RFLP、两个先前鉴定的RFLP（D1S2和D1S57）、两种血型抗原（RH和FY）以及同工酶PGM1构建了1号染色体短臂的连锁图谱。所有27个基因座形成了一个从FY到PND的连续连锁群，在男性中跨度为102厘摩（cM），在女性中为230厘摩。该图谱为1号染色体短臂的大部分区域进行高信息量的多点定位研究提供了基础。
