FGR原癌基因在人类1号染色体短臂遗传连锁图谱上的定位。

Localization of the FGR protooncogene on the genetic linkage map of human chromosome 1p.

作者信息

Dracopoli N C, Stanger B Z, Lager M, Housman D E

机构信息

Center for Cancer Research, Massachusetts Institute of Technology, Cambridge 02139.

出版信息

Genomics. 1988 Aug;3(2):124-8. doi: 10.1016/0888-7543(88)90142-5.

DOI:10.1016/0888-7543(88)90142-5

PMID:2906322

Abstract

A restriction fragment length polymorphism (RFLP) at the human FGR gene, a member of the src family of protooncogenes, has been identified and used to locate FGR on the genetic linkage map of human chromosome 1p. Single-copy sequences subcloned from a cosmid containing the human FGR gene were used to screen a panel of genomic DNAs for RFLPs. One plasmid, designated pB8, detected a high-frequency EcoRI RFLP (allele frequencies, 0.57/0.43). Analysis of a panel of somatic cell hybrids demonstrated that pB8 maps to the region 1p31-pter. Genetic linkage analysis of the 40 families provided by the Centre d'Etude du Polymorphisme Humain (CEPH) showed that FGR maps to a location 3.1 cM from the Rh blood group locus (RH), and falls in the 17.5-cM gap between alpha-fucosidase (FUCA1) and D1S57. The relative gene order of RH and FGR could not be determined unequivocally, but the most favored gene order was 1pter-PND-ALPL-FUCA1-FGR-RH-D1S57-MYCL.

摘要

已鉴定出人类原癌基因src家族成员FGR基因的限制性片段长度多态性（RFLP），并将其用于将FGR定位到人类染色体1p的遗传连锁图谱上。从含有人类FGR基因的黏粒亚克隆的单拷贝序列用于筛选基因组DNA文库以寻找RFLP。一个名为pB8的质粒检测到高频EcoRI RFLP（等位基因频率为0.57/0.43）。对一组体细胞杂种的分析表明，pB8定位于1p31-pter区域。对人类多态性研究中心（CEPH）提供的40个家系进行的遗传连锁分析表明，FGR定位于距Rh血型位点（RH）3.1 cM的位置，位于α-岩藻糖苷酶（FUCA1）和D1S57之间17.5 cM的间隔内。RH和FGR的相对基因顺序无法明确确定，但最可能的基因顺序是1pter-PND-ALPL-FUCA1-FGR-RH-D1S57-MYCL。