Department of Gynecology, Shandong Maternal and Child Health Care Hospital, Jinan, Shandong, China.
Eur Rev Med Pharmacol Sci. 2017 Sep;21(18):4045-4049.
miR-329-3p has been reported to serve as a tumor suppressor in the progression of cervical cancer (CC). The aim of this study was to investigate the clinical significance of miR-329-3p in human CC.
Quantitative RT-PCR (qRT-PCR) was used to detect miR-329-3p expression in CC tissue samples and matched normal cervical tissues. The x2 test was used to analyze the association between miR-329-3p expression and clinical features of CC patients. Moreover, we evaluated the prognostic value of miR-329-3p by Kaplan-Meier survival curve and Cox regression model.
We found that the mean expression level of miR-329-3p in CC tissues was significantly lower than the mean level in the adjacent normal tissues samples (p < 0.01). MiR-329-3p level was significantly associated with lymph node metastasis (p = 0.013), FIGO stage (p = 0.024) and distant metastasis (p = 0.001). Furthermore, a significant difference was found, that CC patients with low miR-329-3p expression level had distinctly shorter overall survival than patients with high miR-329-3p expression level (p = 0.001). Finally, multivariate analyses indicated that miR-329-3p represented an independent predictor for overall survival of CC (p = 0.04).
These results indicated, for the first time, that down-regulation of miR-329-3p was associated with poor prognosis in CC patients. MiR-329-3p can be used as an independent factor to predict survival of patients with CC.
已有研究报道 miR-329-3p 在宫颈癌（CC）的进展中起抑癌作用。本研究旨在探讨 miR-329-3p 在人 CC 中的临床意义。
采用实时定量 RT-PCR（qRT-PCR）检测 CC 组织标本及其配对的正常宫颈组织中 miR-329-3p 的表达。x2 检验分析 miR-329-3p 表达与 CC 患者临床特征的关系。此外，我们通过 Kaplan-Meier 生存曲线和 Cox 回归模型评估 miR-329-3p 的预后价值。
我们发现 CC 组织中 miR-329-3p 的平均表达水平明显低于相邻正常组织样本（p<0.01）。miR-329-3p 水平与淋巴结转移（p=0.013）、FIGO 分期（p=0.024）和远处转移（p=0.001）显著相关。此外，我们发现 miR-329-3p 低表达的 CC 患者总生存期明显短于 miR-329-3p 高表达的患者（p=0.001）。最后，多因素分析表明 miR-329-3p 是 CC 总生存期的独立预测因素（p=0.04）。
这些结果首次表明，miR-329-3p 的下调与 CC 患者的不良预后相关。miR-329-3p 可作为独立因素预测 CC 患者的生存。
