State Key Laboratory of Organic-Inorganic Composites, Beijing University of Chemical Technology, Beijing 100029, China; Research Centre of the Ministry of Education for High Gravity Engineering and Technology, Beijing University of Chemical Technology, Beijing 100029, China.
State Key Laboratory of Organic-Inorganic Composites, Beijing University of Chemical Technology, Beijing 100029, China.
J Colloid Interface Sci. 2018 Feb 1;511:243-250. doi: 10.1016/j.jcis.2017.10.017. Epub 2017 Oct 7.
We report a simple, low cost and environmentally friendly method to prepare NaYF:Yb, Tm upconversion microphosphors (UCMPs) by thermal decomposition of rare earth-trifluoroacetate precursors using paraffin as the high boiling non-coordinating solvent. The UCMPs exhibited cubic phase with defined shape and bright upconversion luminescence. After coating with amphiphilic polymers of phospholipid-polyethylene glycol, the NaYF:Yb, Tm UCMPs were highly dispersed in aqueous solutions and exhibited low cytotoxicity. Furthermore, we explored the use of the micro-injected micro-sized NaYF:Yb, Tm particles for converting of near infrared into blue light in mice brain. The in vivo macroscopic upconversion luminescence imaging results showed that UCMPs located at 1mm depth in the brain could be clearly distinguished. Microscopic upconversion luminescence imaging of the brain sections in vitro revealed that the UCMPs embedded at the particular location in brain tissues of mice were stable without significant diffusion in two weeks.
我们报告了一种简单、低成本且环保的方法，通过使用石蜡作为高热稳定的非配位溶剂，通过热分解稀土三氟乙酸酯前体制备 NaYF:Yb，Tm 上转换微磷（UCMPs）。UCMPs 呈现出具有明确定形和明亮上转换发光的立方相。用两亲聚合物磷脂-聚乙二醇进行涂层后，NaYF:Yb，Tm UCMPs 在水溶液中高度分散，表现出低细胞毒性。此外，我们探索了使用微注射微尺寸的 NaYF:Yb，Tm 颗粒将近红外光转换为小鼠大脑中的蓝光。体内宏观上转换发光成像结果表明，可清楚地区分位于大脑 1mm 深度处的 UCMPs。体外对小鼠脑组织中特定位置嵌入的 UCMPs 的微观上转换发光成像显示，在两周内 UCMPs 没有明显扩散，保持稳定。
