Scharpé S, De Meester I, Vanhoof G, Hendriks D, van Sande M, Van Camp K, Yaron A
Faculty of Medicine, University of Antwerp, Wilrijk, Belgium.
Clin Chem. 1988 Nov;34(11):2299-301.
A new fluorometric assay for determining dipeptidyl peptidase IV (DPP IV; EC 3.4.14.5) was developed. The synthetic substrate glycyl-L-proline-4-methoxy-2-naphthylamide (20 mmol/L), Tris buffer (50 mmol/L, pH 8.3), and serum (20 microL) are mixed and incubated. The reaction is stopped with citrate (100 mmol/L, pH 4.0) and the released 4-methoxy-2-naphthylamine is measured fluorometrically. The mean value of DPP IV activity in serum for 64 healthy subjects was 58 (SD 16) mumol of 4-methoxy-2-naphthylamine released per liter of serum per minute. The proposed procedure is sensitive, rapid, and accurate and can easily be automated.
开发了一种用于测定二肽基肽酶IV（DPP IV；EC 3.4.14.5）的新型荧光测定法。将合成底物甘氨酰-L-脯氨酸-4-甲氧基-2-萘酰胺（20 mmol/L）、Tris缓冲液（50 mmol/L，pH 8.3）和血清（20 μL）混合并孵育。用柠檬酸盐（100 mmol/L，pH 4.0）终止反应，并通过荧光法测量释放的4-甲氧基-2-萘胺。64名健康受试者血清中DPP IV活性的平均值为每分钟每升血清释放58（标准差16）μmol的4-甲氧基-2-萘胺。所提出的方法灵敏、快速且准确，并且可以很容易地实现自动化。
