College of Horticulture, Nanjing Agricultural University, Tongwei Road 6, Nanjing, 210095, P.R. China.
Sci Rep. 2017 Oct 13;7(1):13134. doi: 10.1038/s41598-017-13464-3.
Grapevine is an important and extensively grown fruit crop, which is severely hampered by drought worldwide. So, comprehending the impact of drought on grapevine genetic resources is necessary. In the present study, RNA-sequencing was executed using cDNA libraries constructed from both drought-stress and control plants. Results generated 12,451 differentially expressed genes (DEGs), out of which 8,021 genes were up-regulated, and 4,430 were down-regulated. Further physiological and biochemical investigations were also performed to validate the biological processes associated with the development of grapevine in response to drought stress. Results also revealed that decline in the rate of stomatal conductance, in turn, decrease the photosynthetic activity and CO assimilation in the grapevine leaves. Reactive oxygen species, including stress enzymes and their related proteins, and secondary metabolites were also activated in the present study. Likewise, various hormones also induced in response to drought stress. Overall, the present study concludes that these DEGs play both positive and negative roles in drought tolerance by regulating various biological pathways of grapevine. Nevertheless, our findings have provided valuable gene information for future studies of abiotic stress in grapevine and various other fruit crops.
葡萄是一种重要的、广泛种植的水果作物，但其在全球范围内严重受到干旱的影响。因此，了解干旱对葡萄遗传资源的影响是必要的。本研究使用来自干旱胁迫和对照植物的 cDNA 文库进行 RNA 测序，共生成了 12451 个差异表达基因（DEGs），其中 8021 个基因上调，4430 个基因下调。进一步的生理生化研究也验证了与葡萄对干旱胁迫反应相关的生物过程。结果还表明，气孔导度的下降会降低葡萄叶片的光合作用和 CO2 同化。本研究还激活了活性氧，包括应激酶及其相关蛋白和次生代谢物。同样，各种激素也响应干旱胁迫而被诱导。总的来说，本研究表明这些 DEGs 通过调节葡萄的各种生物途径，在耐旱性中发挥着积极和消极的作用。然而，我们的研究结果为葡萄和其他各种水果作物的非生物胁迫的未来研究提供了有价值的基因信息。
