Department of Orthopaedics, The Fourth Affiliated Hospital of Nantong University, Yancheng, 224005, People's Republic of China.
Department of Orthopaedics, The Fourth Affiliated Hospital of Nantong University, Yancheng, 224005, People's Republic of China.
Biomed Pharmacother. 2017 Dec;96:426-433. doi: 10.1016/j.biopha.2017.10.011. Epub 2017 Oct 12.
More and more researches have shown the vital functions of long non-coding RNAs (LncRNAs) in modulating the biological progresses in tumorigenesis. OR3A4 is such a type of lncRNA. The long non-coding RNA (lncRNA) OR3A4 has recently been exposed to be exceptionally expressed and referred to the development of several cancers. Nevertheless, the biological function and clinical significance of OR3A4 in the carcinogenesis of breast cancer (BC) are still blurry. Therefore, we carried out the following study to make sure the underlying functions of OR3A4 in breast cancer. In this article, by means of qRT-PCR assay, we found that OR3A4 was over-expressed in human breast cancer tissues and cell lines and associated with clinicopathological characteristics. In addition, we also found that the silence of OR3A4 suppressed the proliferation of breast cancer cells by influencing cell cycle and apoptosis, and repressed the cell metastasis via EMT, which was contrary to the results in cells transfected with pcDNA-OR3A4. And all the functions of OR3A4 in breast cancer were realized in the transcriptional level according to the subcellular fractionation location assay. Thus, based upon all of the data above, our study might provide a better understanding of the pathogenesis of breast cancer and implied that OR3A4 might be a potent prognostic and therapeutic target in breast cancer.
越来越多的研究表明长非编码 RNA（LncRNAs）在调节肿瘤发生中的生物学进程中具有重要功能。OR3A4 就是这样一种 lncRNA。长非编码 RNA（lncRNA）OR3A4 最近被发现异常表达，并与几种癌症的发展有关。然而，OR3A4 在乳腺癌（BC）发生中的生物学功能和临床意义仍不清楚。因此，我们进行了以下研究，以确定 OR3A4 在乳腺癌中的潜在功能。在本文中，通过 qRT-PCR 检测，我们发现 OR3A4 在人乳腺癌组织和细胞系中过度表达，并与临床病理特征相关。此外，我们还发现，OR3A4 的沉默通过影响细胞周期和细胞凋亡抑制乳腺癌细胞的增殖，并通过 EMT 抑制细胞转移，这与转染 pcDNA-OR3A4 的细胞的结果相反。根据亚细胞分馏位置检测，OR3A4 在乳腺癌中的所有功能都是在转录水平上实现的。因此，基于上述所有数据，我们的研究可能为乳腺癌的发病机制提供了更好的理解，并暗示 OR3A4 可能是乳腺癌有潜力的预后和治疗靶点。
