Chen Chengze, Xia Erjie, Bhandari Adheesh, Wang Yinghao, Shen Yanyan, Sindan Namita, Lin Yuehlung, Wang Xiaoshang, Yang Fan, Wang Ouchen
Department of Thyroid and Breast Surgery, The First Affiliated Hospital of Wenzhou Medical University Wenzhou, Zhejiang, PR China.
Department of Reproductive Health Center, The Second Affiliated Hospital of Wenzhou Medical University Wenzhou, Zhejiang, People's Republic of China.
Int J Clin Exp Pathol. 2018 Mar 1;11(3):1453-1459. eCollection 2018.
Many long non-coding RNAs (lncRNAs) have been found to exert influences on biological processes including tumorigenesis. Many lncRNAs have been reported as potential therapeutic targets and prognostic biomarkers in multiple cancers. CCND2 antisense RNA 1 (CCND2-AS1) is an lncRNA recently reported to be involved in the progression of glioma cancers. However, whether CCND2-AS1 is associated with progression of breast cancer remains unknown. In this study, quantitative real-time polymerase chain reaction (qRT-PCR) was used to measure CCND2-AS1 gene expression in breast cancer cell in lines. CCND2-AS1 expression was significantly over-expressed compared to normal breast epithelial cells. Gain-and loss-of-function experiments were performed to investigate the role of CCND2-AS1, where we found that CCND2-AS1 knockdown in MDA-MB-231 significantly suppressed cell proliferation, migration, and invasion. In contrast, CCND2-AS1 overexpression in BT-549 had the opposite effects. Our findings indicate that lncRNA CCND2-AS1 is a gene associated with breast cancer and might become a potential therapeutic target.
许多长链非编码RNA（lncRNAs）已被发现对包括肿瘤发生在内的生物学过程产生影响。许多lncRNAs已被报道为多种癌症中的潜在治疗靶点和预后生物标志物。细胞周期蛋白D2反义RNA 1（CCND2-AS1）是一种最近报道与神经胶质瘤癌进展有关的lncRNA。然而，CCND2-AS1是否与乳腺癌进展相关仍不清楚。在本研究中，采用定量实时聚合酶链反应（qRT-PCR）来检测乳腺癌细胞系中CCND2-AS1基因的表达。与正常乳腺上皮细胞相比，CCND2-AS1的表达显著上调。进行了功能获得和功能缺失实验以研究CCND2-AS1的作用，我们发现MDA-MB-231细胞中CCND2-AS1的敲低显著抑制了细胞增殖、迁移和侵袭。相反，BT-549细胞中CCND2-AS1的过表达则产生相反的效果。我们的研究结果表明，lncRNA CCND2-AS1是一种与乳腺癌相关的基因，可能成为潜在的治疗靶点。
