Department of Development and Regeneration, KU Leuven, Leuven, Belgium.
Department of Development and Regeneration, KU Leuven, Leuven, Belgium.
J Minim Invasive Gynecol. 2018 May-Jun;25(4):578-579. doi: 10.1016/j.jmig.2017.10.008. Epub 2017 Oct 13.
To demonstrate how a novel laparoscopic approach allows the development of a mouse model for endometriosis after seeding menstrual endometrium from donor mice into the abdominal cavity of syngeneic recipient mice.
A step-by-step video description of the techniques used to adapt the estrous cycle of mice towards a menstrual cycle and to subsequently induce endometriosis via laparoscopic seeding of menstrual endometrium.
University research institute.
All animal experiments were ethically approved by KU Leuven, Belgium (ethical approval number: P031/2013).
INTERVENTIONS, MEASUREMENTS, AND MAIN RESULTS: Oophorectomized female C57BL/6JRj mice received a series of estrogen injections. Next, a progesterone pellet was administered, together with a second series of estrogen injections. In addition, decidualization of the endometrium was induced with an intrauterine sesame oil stimulus. Four days later the progesterone pellet was removed and menstruation started [1]. Five hours after the progesterone pellet was removal the uterus was harvested, and the menstrual endometrium was dissected and seeded into the abdominal cavity of syngeneic recipient mice to induce endometriosis [2] using a laparoscopic approach [3]. Uterus and lesions were removed from the recipient mice 1 week after induction, and tissues were immunohistochemically stained for H&E, vimentin, and cytokeratin.
In this video we show a novel methodology to induce endometriosis in mice using laparoscopic inoculation of syngeneic menstrual endometrium, mimicking Sampson's theory of retrograde menstruation [4]. Compared with currently available rodent models, our model offers a less invasive and more physiologic way for fundamental and preclinical endometriosis research, with a high endometriosis incidence and lesion take rate.
展示一种新的腹腔镜方法，通过将供体小鼠的月经子宫内膜播种到同基因受体小鼠的腹腔中，来开发子宫内膜异位症的小鼠模型。
对适应小鼠动情周期并随后通过腹腔镜播种月经子宫内膜来诱导子宫内膜异位症的技术进行分步视频描述。
大学研究所。
所有动物实验均获得比利时鲁汶天主教大学（KU Leuven）的伦理批准（伦理批准编号：P031/2013）。
干预措施、测量和主要结果：去卵巢雌性 C57BL/6JRj 小鼠接受一系列雌激素注射。然后，给予孕酮丸，并给予第二系列雌激素注射。此外，用宫内芝麻油刺激诱导子宫内膜蜕膜化。4 天后取出孕酮丸，开始月经[1]。取出孕酮丸后 5 小时收获子宫，将月经子宫内膜解剖并播种到同基因受体小鼠的腹腔中，使用腹腔镜方法[3]诱导子宫内膜异位症[2]。诱导后 1 周从受体小鼠中取出子宫和病变，并用 H&E、波形蛋白和细胞角蛋白进行免疫组织化学染色。
在本视频中，我们展示了一种使用腹腔镜接种同基因月经子宫内膜在小鼠中诱导子宫内膜异位症的新方法，模拟了 Sampson 的逆行月经理论[4]。与现有的啮齿动物模型相比，我们的模型为基础和临床前子宫内膜异位症研究提供了一种侵入性更小、更符合生理的方法，具有较高的子宫内膜异位症发生率和病变接种率。
