Shu S, Fan Q B, Lang J H
Department of Obstetrics and Gynecology, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100730, China.
Zhonghua Fu Chan Ke Za Zhi. 2019 Aug 25;54(8):527-533. doi: 10.3760/cma.j.issn.0529-567x.2019.08.005.
To investigate the feasibility of a non-invasive sampling method by collecting menstrual blood and obtaining endometrium for further research in menstruation-related diseases. On the second day of menstruation, menstrual blood was collected with menstrual cups for 4 hours, and the menstrual endometrium was filtered through a metal screen for weighing, cryopreserved, immunohistochemical staining and cell culture. The collection process was painless and non-invasive. In the control group, the menstrual volume was (9.1±0.7) ml, and the endometrial tissue weight was (91.0±14.7) g. In the endometriosis group, the menstrual volume was (9.6±1.9) ml (0.022), and the endometrial tissue weight was (134.7±43.9) g (0.057). Endometrial cell culture was successful in all patients and should not be contaminated. The growth curve was a finite cell line type. The expression of cytokeratin 19 and vimentin in menstrual endometrium and cells were positive. By collecting menstrual blood and filtering endometrial tissue, it is an ideal non-invasive sampling method. In combination with advanced experimental technology, menstrual endometrium make further researches of endometriosis, endometrial lesions or other menstruation-related diseases possible.
为研究通过收集月经血获取子宫内膜用于与月经相关疾病进一步研究的非侵入性采样方法的可行性。在月经第二天，用月经杯收集月经血4小时，将月经子宫内膜通过金属筛网过滤以称重、冷冻保存、进行免疫组织化学染色和细胞培养。收集过程无痛且非侵入性。对照组中，月经量为（9.1±0.7）ml，子宫内膜组织重量为（91.0±14.7）g。子宫内膜异位症组中，月经量为（9.6±1.9）ml（P = 0.022），子宫内膜组织重量为（134.7±43.9）g（P = 0.057）。所有患者的子宫内膜细胞培养均成功且未被污染。生长曲线为有限细胞系类型。月经子宫内膜和细胞中细胞角蛋白19和波形蛋白的表达呈阳性。通过收集月经血和过滤子宫内膜组织，这是一种理想的非侵入性采样方法。结合先进的实验技术，月经子宫内膜使得对子宫内膜异位症、子宫内膜病变或其他与月经相关疾病的进一步研究成为可能。
