Ghafelehbashi H, Pahlevan Kakhki M, Kular L, Moghbelinejad S, Ghafelehbashi S H
Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, Iran.
Department of Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran.
Scand J Immunol. 2017 Dec;86(6):479-485. doi: 10.1111/sji.12620.
Although aberrant expression of cytokines such as IL-1B and IFNG in blood from psychiatric patients supports a role of inflammation in the pathogenesis of the disease, little is known about mechanisms underlying their regulation. We aimed to evaluate the putative role of IFNG-AS1 long non-coding RNA (lncRNA) in controlling of IFNG locus in patients with schizophrenia (SZ) and bipolar (BP). We analysed the expression levels of IFNG-AS1 long non-coding RNA, and IFNG and IL-1B mRNAs in blood cells from 27 SZ- and 30 BP-medicated patients and in 32 healthy controls. Our data showed that IFNG-AS1 expression dramatically decreased in BP and SZ patients compared with controls and was significantly correlated with IFNG expression in patients specifically. Transcript levels of IL-1B were also significantly reduced in BP and SZ patients compared with controls. No significant differences in the expression of IFNG-AS1, IFNG and IL-1B genes were found between patients with BP and SZ. Our data shed further light on the potential role of inflammation, and more particularly inflammatory lncRNAs, in SZ and BP diseases and their pharmacological treatment.
尽管精神病患者血液中白细胞介素-1β（IL-1B）和干扰素-γ（IFNG）等细胞因子的异常表达支持炎症在该疾病发病机制中的作用，但对其调控机制知之甚少。我们旨在评估IFNG反义RNA1（IFNG-AS1）长链非编码RNA（lncRNA）在精神分裂症（SZ）和双相情感障碍（BP）患者中对IFNG基因座的调控作用。我们分析了27例接受药物治疗的SZ患者、30例接受药物治疗的BP患者以及32名健康对照者血细胞中IFNG-AS1长链非编码RNA、IFNG和IL-1B mRNA的表达水平。我们的数据显示，与对照组相比，BP和SZ患者中IFNG-AS1的表达显著降低，且在患者中与IFNG表达显著相关。与对照组相比，BP和SZ患者中IL-1B的转录水平也显著降低。在BP和SZ患者之间，未发现IFNG-AS1、IFNG和IL-1B基因表达存在显著差异。我们的数据进一步揭示了炎症，尤其是炎症性lncRNAs，在SZ和BP疾病及其药物治疗中的潜在作用。
