Yamamoto Satoshi, Okamoto Hiroyuki, Haga Makoto, Shigematsu Kunihiro, Miyata Tetsuro, Watanabe Toshiaki, Ogawa Yoko, Takagi Yoshihide, Asakura Tetsuo
Division of Vascular Surgery, Department of Surgery, Graduate School of Medicine, The University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo 113-8655, Japan.
J Mater Chem B. 2016 Feb 7;4(5):938-946. doi: 10.1039/c5tb02528a. Epub 2016 Jan 11.
The histological effects of silk fibroin (SF) in vascular grafts have not been clarified comprehensively in a large-animal model. This study aimed to observe the histological changes in vascular grafts by using Bombyx mori SF in a dog model. A splice graft consisting of SF and control grafts were implanted in the abdominal aorta of dogs, and the histological characteristics of the 2 types of grafts in each splice graft were compared. Five splice grafts consisted of one graft made of polyester (PE) fibers coated with SF and another coated with gelatin, 4 splice grafts consisted of one graft made of SF fibers coated with gelatin and another made of PE fibers coated with gelatin, and 2 splice grafts consisted of one graft made of SF fibers coated with SF and another made of PE fibers coated with SF. The graft made of PE fibers coated with SF showed more endothelial cells than the graft made of PE fibers coated with gelatin. The grafts using SF as a coating material or graft fibers showed a thinner luminal layer than the grafts made of PE fibers coated with gelatin. This study suggests that SF use for vascular grafts has advantages of rapid endothelialization and tendency to form thin luminal layers.
丝素蛋白（SF）在大动物模型的血管移植物中的组织学效应尚未得到全面阐明。本研究旨在通过在犬模型中使用家蚕丝素蛋白观察血管移植物的组织学变化。将由丝素蛋白组成的拼接移植物和对照移植物植入犬的腹主动脉，并比较每个拼接移植物中两种移植物的组织学特征。5个拼接移植物由一个涂有丝素蛋白的聚酯（PE）纤维制成的移植物和另一个涂有明胶的移植物组成，4个拼接移植物由一个涂有明胶的丝素蛋白纤维制成的移植物和另一个涂有明胶的PE纤维制成的移植物组成，2个拼接移植物由一个涂有丝素蛋白的丝素蛋白纤维制成的移植物和另一个涂有丝素蛋白的PE纤维制成的移植物组成。涂有丝素蛋白的PE纤维制成的移植物比涂有明胶的PE纤维制成的移植物显示出更多的内皮细胞。使用丝素蛋白作为涂层材料或移植物纤维的移植物比涂有明胶的PE纤维制成的移植物显示出更薄的管腔层。本研究表明，丝素蛋白用于血管移植物具有内皮化迅速和形成薄管腔层的趋势的优点。
