Hyun Ji-Chul, Choi Jongchan, Jung Yu-Gyung, Yang Sung
School of Mechanical Engineering, Gwangju Institute of Science and Technology (GIST), Gwangju 500-712, South Korea.
Department of Biomedical Science and Engineering, Gwangju Institute of Science and Technology (GIST), Gwangju 500-712, South Korea.
Biomicrofluidics. 2017 Sep 27;11(5):054108. doi: 10.1063/1.5005612. eCollection 2017 Sep.
In this study, a microfluidic cell concentrator with a reduced-deviation-flow herringbone structure is proposed. The reduced-deviation-flow herringbone structure reduces the magnitude of deviation flow by a factor of 3.3 compared to the original herringbone structure. This structure shows higher recovery efficiency compared to the original herringbone structure for various particle sizes at high flow rate conditions. Using the reduced-deviation-flow herringbone structure, the experimental results show a recovery efficiency of 98.5% and a concentration factor of 3.4× at a flow rate of 100 ml/h for all particle sizes. An iterative concentration process is performed to achieve a higher concentration factor for 10.2-m particles and Jurkat cells. With two stages of the concentration process, we were able to achieve over 98% recovery efficiency and a concentration factor of 10-11×. Cell viability was found to be above 96% after iterative concentration. We believe that this device could be used to concentrate cells as a preparatory step for studying low-abundance cells.
在本研究中，提出了一种具有减小偏差流鱼骨结构的微流控细胞浓缩器。与原始鱼骨结构相比，减小偏差流鱼骨结构将偏差流的幅度降低了3.3倍。在高流速条件下，对于各种粒径的粒子，该结构显示出比原始鱼骨结构更高的回收效率。使用减小偏差流鱼骨结构，实验结果表明，在流速为100 ml/h时，所有粒径的粒子的回收效率为98.5%，浓缩系数为3.4倍。对10.2微米的粒子和Jurkat细胞进行迭代浓缩过程以实现更高的浓缩系数。经过两个阶段的浓缩过程，我们能够实现超过98%的回收效率和10-11倍的浓缩系数。迭代浓缩后发现细胞活力高于96%。我们认为，该装置可用于浓缩细胞，作为研究低丰度细胞的预处理步骤。
