Li An-Zhang, Lin Long-Zhen, Zhang Ming-Xia, Zhu Hong-Hui
State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Microbial Culture Collection Center (GDMCC), Guangdong Institute of Microbiology, Guangzhou 510070, PR China.
Int J Syst Evol Microbiol. 2017 Dec;67(12):5056-5061. doi: 10.1099/ijsem.0.002413. Epub 2017 Oct 16.
A novel bacterium, designated R14M6, was isolated from deep-sea sediment collected from the Ross Sea, Antarctica. Cells are Gram-stain-negative, aerobic, pale yellow, short-rod-shaped, polar-flagellated and aggregate-forming. Growth occurs at 4-36 °C, pH 6.0-8.3, and in 1-15 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain R14M6 clustered together with Aurantimonas endophytica EGI6500337 and fell within the genus Aurantimonas. The 16S rRNA gene sequence of strain R14M6 shared similarity with A. endophytica EGI6500337 (99.15 %), A. manganoxydans DSM 21871 (97.73 %), A. coralicida DSM 14790 (97.58 %) and 'A. litoralis' KCTC 12094 (97.51 %). The DNA-DNA relatedness values between strain R14M6 and A. endophytica EGI6500337, A. coralicida DSM 14790, A. manganoxydans DSM 21871 and 'A. litoralis' KCTC 12094 were 36.9±4.5, 27.6±2.8, 29.6±1.2 and 25.2±2.4 % respectively. The major fatty acid of strain R14M6 was C18 : 1ω7c. The major polar lipids were phosphatidylcholine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and an unidentified aminolipid. Strain R14M6 contained Q-10 as the dominant isoprenoid quinone. The DNA G+C content of strain R14M6 was 67.4 mol%. Based on the phylogenetic, physiological and chemotaxonomic analyses, strain R14M6 represents a novel species of the genus Aurantimonas, for which the name Aurantimonas aggregata sp. nov. is proposed. The type strain is R14M6 (=GDMCC 1.1202=KCTC 52919).
从南极罗斯海采集的深海沉积物中分离出一种新型细菌，命名为R14M6。细胞革兰氏染色阴性，需氧，浅黄色，短杆状，具极鞭毛且能形成聚集体。该菌在4-36 °C、pH 6.0-8.3以及1-15 %（w/v）NaCl条件下生长。基于16S rRNA基因序列的系统发育分析表明，菌株R14M6与内生橙色单胞菌EGI6500337聚在一起，属于橙色单胞菌属。菌株R14M6的16S rRNA基因序列与内生橙色单胞菌EGI6500337（99.15 %）、氧化锰橙色单胞菌DSM 21871（97.73 %）、珊瑚橙色单胞菌DSM 14790（97.58 %）和“滨海橙色单胞菌”KCTC 12094（97.51 %）具有相似性。菌株R14M6与内生橙色单胞菌EGI6500337、珊瑚橙色单胞菌DSM 14790、氧化锰橙色单胞菌DSM 21871和“滨海橙色单胞菌”KCTC 12094的DNA-DNA杂交值分别为36.9±4.5、27.6±2.8、29.6±1.2 和25.2±2.4 %。菌株R14M6的主要脂肪酸为C18 : 1ω7c。主要极性脂为磷脂酰胆碱、双磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺和一种未鉴定的氨基脂。菌株R14M6以Q-10作为主要类异戊二烯醌。菌株R14M6的DNA G+C含量为67.4 mol%。基于系统发育、生理和化学分类分析，菌株R14M6代表橙色单胞菌属的一个新物种，为此提出新名称聚集体橙色单胞菌（Aurantimonas aggregata sp. nov.）。模式菌株为R14M6（=GDMCC 1.1202=KCTC 52919）。
