School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing, 100083, China.
Shanghai-MOST Key Laboratory of Health and Disease Genomics, Chinese National Human Genome Center At Shanghai, Shanghai, 201203, China.
Arch Microbiol. 2021 Mar;203(2):609-620. doi: 10.1007/s00203-020-02058-9. Epub 2020 Sep 29.
Upland rice is an ecotype crop resulting from the long-term domestication and evolution of rice in dry land without a water layer. Generally, the stems and leaves are thick and luxuriant, while the leaves also typically broad and light. The root system is developed with abundant root hair, and the osmotic pressure of the root and cell juice concentration in the leaves is high, while this plant is drought-resistant, heat-resistant, and water absorbent. This study aims to reveal the "core flora" of the endophytes in upland rice seeds by examining their diversity and community structures. It further intends to reveal the impact of the soil environment on the formation of endophyte community structures in upland rice seeds by comparing the environmental soil microorganisms in upland rice habitats. In this study, high-throughput sequencing technology based on the Illumina Hiseq 2500 platform was used to investigate the structure and diversity of endophytic bacterial communities using upland rice varieties collected from different locations and soil samples from unified planting sites as materials. Here, 42 endophytic OTUs were found to coexist in the 14 samples. At the phylum level, the first dominant phyla in all the samples were Proteobacteria (93.81-99.99%). At the genus level, Pantoea (8.77-87.77%), Pseudomonas (1.15-61.58%), Methylobacterium (0.40-4.64%), Sphingomonas (0.26-3.85%), Microbacterium (0.01-4.67%) and Aurantimonas (0.04-4.34%), which represent the core microflora in upland rice seeds, served as the dominant genera that coexisted in all the upland rice seeds tested. This study significant for the isolation, screening, functional evaluation, and re-action of various functional microorganisms in upland rice to improve its agronomic traits. It also provides a specific reference for the interaction between microorganisms and plants.
旱稻是一种在旱地生长、无水分层的条件下，经过长期驯化和演化而形成的生态型作物。旱稻一般茎、叶粗壮繁茂，叶片也通常宽大而浅，根系发达，根毛丰富，叶片和细胞液的渗透压高，具有较强的抗旱、耐热和吸水能力。本研究旨在通过检测其多样性和群落结构，揭示旱稻种子内生菌的“核心菌群”。并通过比较旱稻生境的土壤环境与微生物，进一步揭示土壤环境对旱稻种子内生菌群落结构形成的影响。本研究以不同地区采集的旱稻品种和统一种植地点的土壤样本为材料，采用基于 Illumina Hiseq 2500 平台的高通量测序技术，调查了旱稻内生细菌群落的结构和多样性。结果在 14 个样本中共发现 42 个内生 OTU 共存。在门水平上，所有样本的第一优势门均为变形菌门（93.81%-99.99%）。在属水平上，丰度最高的属为 Pantoea（8.77%-87.77%）、Pseudomonas（1.15%-61.58%）、Methylobacterium（0.40%-4.64%）、Sphingomonas（0.26%-3.85%）、Microbacterium（0.01%-4.67%）和 Aurantimonas（0.04%-4.34%），这些代表旱稻种子核心微生物群的属在所有旱稻种子中共同存在，是优势共存属。本研究为旱稻中各种功能微生物的分离、筛选、功能评价和反应提供了重要依据，以提高其农艺性状，也为微生物与植物的相互作用提供了具体参考。
