Hasegawa Daniel K, Chen Wenbo, Zheng Yi, Kaur Navneet, Wintermantel William M, Simmons Alvin M, Fei Zhangjun, Ling Kai-Shu
USDA-ARS, US Vegetable Laboratory, Charleston, South Carolina, USA; Boyce Thompson Institute, Ithaca, New York, USA.
Boyce Thompson Institute, Ithaca, New York, USA.
Virology. 2018 Jan 1;513:52-64. doi: 10.1016/j.virol.2017.10.008. Epub 2017 Oct 13.
The whitefly Bemisia tabaci can transmit hundreds of viruses to numerous agricultural crops in the world. Five genera of viruses, including Begomovirus and Crinivirus, are transmitted by B. tabaci. There is little knowledge about the genes involved in virus acquisition and transmission by whiteflies. Using a comparative transcriptomics approach, we evaluated the gene expression profiles of whiteflies (B. tabaci MEAM1) after feeding on tomato infected by a begomovirus, Tomato yellow leaf curl virus (TYLCV), in comparison to a recent study, in which whiteflies were fed on tomato infected by the crinivirus, Tomato chlorosis virus (ToCV). The data revealed similar temporal trends in gene expression, but large differences in the number of whitefly genes when fed on TYLCV or ToCV-infected tomato. Transcription factors, cathepsins, receptors, and a hemocyanin gene, which is implicated in mediating antiviral immune responses in other insects and possibly virus transmission, were some of the genes identified.
烟粉虱能将数百种病毒传播给世界上众多的农作物。包括双生病毒属和毛形病毒属在内的五个病毒属可由烟粉虱传播。关于粉虱获取和传播病毒所涉及的基因，人们了解甚少。通过比较转录组学方法，我们评估了烟粉虱（烟粉虱MEAM1）取食感染双生病毒——番茄黄化曲叶病毒（TYLCV）的番茄后的基因表达谱，并与最近一项研究进行了比较，在该研究中，粉虱取食感染了毛形病毒——番茄褪绿病毒（ToCV）的番茄。数据揭示了基因表达中相似的时间趋势，但粉虱取食感染TYLCV或ToCV的番茄时，其基因数量存在很大差异。转录因子、组织蛋白酶、受体以及一个血蓝蛋白基因是鉴定出的部分基因，血蓝蛋白基因在其他昆虫中介导抗病毒免疫反应并可能参与病毒传播。
