The First Affiliated Hospital, Guangzhou University of Chinese Medicine, Guangzhou, 510405, China.
Department of Cardiac Surgery, Cardiovascular Center, The University of Michigan, Ann Arbor, MI, 48109, USA.
Stem Cell Res Ther. 2017 Oct 16;8(1):230. doi: 10.1186/s13287-017-0675-2.
Application of cardiac stem cells combined with biomaterial scaffold is a promising therapeutic strategy for heart repair after myocardial infarction. However, the optimal cell types and biomaterials remain elusive.
In this study, we seeded Isl1 embryonic cardiac progenitor cells (CPCs) into decellularized porcine small intestinal submucosa extracellular matrix (SIS-ECM) to assess the therapeutic potential of Isl1 CPCs and the biocompatibility of SIS-ECM with these cells.
We observed that SIS-ECM supported the viability and attachment of Isl1 CPCs. Importantly, Isl1 CPCs differentiated into cardiomyocytes and endothelial cells 7 days after seeding into SIS-ECM. In addition, SIS-ECM with CPC-derived cardiomyocytes showed spontaneous contraction and responded to β-adrenergic stimulation. Next, patches of SIS-ECM seeded with CPCs for 7 days were transplanted onto the outer surface of infarcted myocardium in mice. Four weeks after transplantation, the patches were tightly attached to the surface of the host myocardium and remained viable. Transplantation of patches improved cardiac function, decreased the left ventricular myocardial scarring area, and reduced fibrosis and heart failure.
Transplantation of Isl1 CPCs seeded in SIS-ECM represents an effective approach for cell-based heart therapy.
心脏干细胞与生物材料支架的联合应用是心肌梗死后心脏修复的一种很有前途的治疗策略。然而，最佳的细胞类型和生物材料仍然难以捉摸。
在这项研究中，我们将 Isl1 胚胎心脏祖细胞（CPCs）接种到脱细胞化的猪小肠黏膜下基质（SIS-ECM）中，以评估 Isl1 CPCs 的治疗潜力和 SIS-ECM 与这些细胞的生物相容性。
我们观察到 SIS-ECM 支持 Isl1 CPCs 的存活和附着。重要的是，Isl1 CPCs 在接种到 SIS-ECM 7 天后分化为心肌细胞和内皮细胞。此外，用 CPC 衍生的心肌细胞接种的 SIS-ECM 显示出自发收缩，并对β-肾上腺素能刺激产生反应。接下来，将接种了 CPC 的 SIS-ECM 贴片在小鼠梗死心肌的外表面移植。移植后 4 周，贴片紧密附着在宿主心肌表面并保持存活。贴片移植改善了心脏功能，减少了左心室心肌瘢痕面积，并减少了纤维化和心力衰竭。
接种在 SIS-ECM 中的 Isl1 CPCs 的移植代表了一种有效的基于细胞的心脏治疗方法。
