UMR Ingénierie Procédés Aliments, AgroParisTech, Inra, Université Paris-Saclay, 91300 Massy, France.
UMR Ingénierie Procédés Aliments, AgroParisTech, Inra, Université Paris-Saclay, 91300 Massy, France.
Food Chem. 2018 Mar 1;242:113-121. doi: 10.1016/j.foodchem.2017.08.108. Epub 2017 Sep 1.
This study attempts at uniting the analysis of four different classes of contaminants for both liquid and solid tea samples. A total of 32 compounds, classified as pesticides, mycotoxins, process-induced toxicants or packaging contaminants, were carefully chosen for their diversity of structures and physicochemical properties. The proposed method combines a sample treatment strategy coming from metabolomics with liquid chromatography analysis using a silica bonded C18-pentafluorophenyl column coupled to high resolution mass spectrometry. For tea brew, dilute and shoot method provides good quantification (70-120% recoveries and <20% RSD) for more than 80% of compounds. For tea leaves, strong matrix effects are observed, thus, matrix-matched calibration is required to reach good performances, i.e. 63% of compounds quantified and 81% detected at 10µg/kg. Finally, method performances were evaluated against existing regulations, and it appears that 69% of contaminants are quantified and 91% detected at levels lower than their respective European regulation limits.
本研究旨在结合分析四类不同的污染物，包括液体和固体茶叶样本。总共选择了 32 种化合物，这些化合物被归类为农药、霉菌毒素、工艺诱导性毒物或包装污染物，它们的结构和物理化学性质具有多样性。该方法结合了代谢组学中的样品处理策略和使用硅胶键合 C18-五氟苯基柱与高分辨率质谱联用的液相色谱分析。对于茶泡，稀释和直接进样方法对 80%以上的化合物进行了良好的定量（70-120%回收率和 <20%RSD）。对于茶叶，观察到强基质效应，因此需要基质匹配校准以达到良好的性能，即 63%的化合物定量，10µg/kg 时 81%的化合物被检测到。最后，根据现有法规评估方法性能，结果表明，69%的污染物在低于各自欧洲法规限量的水平下被定量，91%的污染物被检出。
