Scuola di Ingegneria, Università degli Studi della Basilicata, via dell'Ateneo Lucano, 10, 85100 Potenza, Italy.
Dipartimento di Scienze, Università degli Studi della Basilicata, via dell'Ateneo Lucano, 10, 85100 Potenza, Italy.
Food Chem. 2018 Mar 1;242:497-504. doi: 10.1016/j.foodchem.2017.09.091. Epub 2017 Sep 19.
The present study deals with the evaluation of antidiabetic activities of Fagioli di Sarconi beans (Phaseolus vulgaris), including 21 ecotypes protected by the European Union with the mark PGI (i.e., Protected Geographical Indication), and cultivated in Basilicata (southern Italy). For this purpose, α-glucosidase and α-amylase assays were assessed; among all bean ecotypes, the tight green seed colour of Verdolino extracts exhibited the highest α-glucosidase and α-amylase inhibitory activity with IC=1.1±0.1μg/ml and IC=19.3±1.1μg/ml, respectively. Phytochemical compound screening of all Fagioli di Sarconi beans performed by flow injection-electrospray ionization-ultrahigh resolution mass spectrometry (uHRMS) and based on the calculation of elemental formulas from accurate m/z values, was helpful to annotate specific compounds, such as alkaloids, saponins, flavonoids, and terpenoids, which are most likely responsible for their biological activity.
本研究评估了萨科尼豆（菜豆）的抗糖尿病活性，包括欧盟受 PGI（受保护地理标志）保护的 21 个生态型，这些生态型在意大利南部的巴西利卡塔种植。为此，评估了α-葡萄糖苷酶和α-淀粉酶的活性；在所有豆生态型中，Verdolino 提取物的紧密的绿色种子颜色表现出最高的α-葡萄糖苷酶和α-淀粉酶抑制活性，IC=1.1±0.1μg/ml 和 IC=19.3±1.1μg/ml。通过流动注射-电喷雾电离-超高分辨率质谱（uHRMS）对所有萨科尼豆进行的植物化学化合物筛选，并根据从精确 m/z 值计算的元素公式进行注释，有助于注释特定化合物，如生物碱、皂苷、类黄酮和萜类化合物，这些化合物可能是其生物活性的主要原因。
