Aliaga-Aguilar Hugo
Department of Mechanical Engineering and Materials Science, Yale University, 17 Hillhouse Ave., New Haven, CT, 06511, USA.
Departamento de Ingeniería de Organización, Administración de Empresas y Estadística, Escuela Técnica Superior de Ingeniería Aeronáutica y del Espacio, Universidad Politecnica de Madrid, Plaza del Cardenal Cisneros, 3, 28040, Madrid, Spain.
J Am Soc Mass Spectrom. 2018 Jan;29(1):17-25. doi: 10.1007/s13361-017-1826-5. Epub 2017 Oct 16.
Paper spray ionization has arisen relatively recently as a complement and alternative to electro- and nanospray ionization with silica capillaries. A majority of the work in the present literature focuses on the chemical aspect of paper spray. In order to study the physical and phenomenological facet of its implementation, we measured current and voltage distributions of Taylor cones. To study transport phenomena on filter paper, we addressed the behavior of large, sparingly soluble tetraalkylammonium ions, which are usually used as mobility standards, in paper spray. The variation of intensity with time of monomers and dimers of these ions was measured with a differential mobility analyzer and compared with that produced by contamination in the paper. At the same time, we evaluated the proficiency of different paper spray techniques for protein analysis using nano spray as a reference. Experiments suggest that Taylor cones in paper spray are subject to hysteresis, whereas transport phenomena in the porous substrate notably affects the ionization of the sample. Additionally, we observed that paper spray tends to favor lower charge states in proteins. Graphical Abstract.
纸喷雾电离作为使用硅胶毛细管的电喷雾和纳米喷雾电离的补充及替代方法，是相对较新出现的。当前文献中的大部分工作聚焦于纸喷雾的化学方面。为了研究其实施过程中的物理和现象学层面，我们测量了泰勒锥的电流和电压分布。为了研究滤纸表面的传输现象，我们研究了通常用作迁移率标准的大的、微溶的四烷基铵离子在纸喷雾中的行为。用差分迁移率分析仪测量了这些离子单体和二聚体强度随时间的变化，并与纸张污染产生的变化进行了比较。同时，我们以纳米喷雾为参照，评估了不同纸喷雾技术用于蛋白质分析的效能。实验表明，纸喷雾中的泰勒锥存在滞后现象，而多孔基质中的传输现象显著影响样品的电离。此外，我们观察到纸喷雾倾向于使蛋白质呈现较低的电荷状态。图形摘要。
