Hao Hao Li-Xin, Zhao Xin-Huai
Key Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin, PR China.
Key Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin, PR China / Department of Food Science, Northeast Agricultural University, Harbin, PR China.
Pak J Pharm Sci. 2017 Jul;30(4):1383-1388.
Dioscorea opposita is an edible and medicinal plant available in many areas of China. This study aimed to assess in vitro immune potentials of a water-soluble polysaccharide extract from D. opposita planted in Henan Province, China. In vitro effects of the extract on three immune cells (macrophages, natural killer cells and splenocytes) from mice and secretion of eight immune-related molecules in macrophages and splenocytes were evaluated. In total, the extract exhibited a dose-dependent manner on these immunological responses. The extract at dose level of 50μg/ml enhanced respective splenocyte proliferation, macrophage phagocytosis, and natural killer cell activity by 150%, 18% and 47%, increased secretion of interleukin-2 and interferon-γ (from 41.4 and 24.6 pg/ml to 48.8 and 91.5 pg/ml, respectively) but decreased secretion of interleukin-4 (from 38.9 to 27.9 pg/ml) in splenocytes. The extract at the same dose level also stimulated inducible nitric oxide synthase and lysozyme in macrophages, and enhanced secretion of interleukin-6, interleukin-1β and tumor necrosis factor-α (from 26.6, 73.4 and 39.6 pg/ml to 60.2, 131.0 and 144.7 pg/ml, respectively). It is concluded that water-soluble polysaccharides from D. opposita have immune potentials to the body, via activating immune cells and regulating the secretion of immune-related molecules.
山药是一种在中国许多地区都有分布的药食两用植物。本研究旨在评估来自中国河南省种植的山药水溶性多糖提取物的体外免疫活性。评估了该提取物对小鼠三种免疫细胞（巨噬细胞、自然杀伤细胞和脾细胞）的体外作用以及巨噬细胞和脾细胞中八种免疫相关分子的分泌情况。总体而言，该提取物对这些免疫反应呈剂量依赖性。50μg/ml剂量水平的提取物使脾细胞增殖、巨噬细胞吞噬作用和自然杀伤细胞活性分别提高了150%、18%和47%，脾细胞中白细胞介素-2和干扰素-γ的分泌增加（分别从41.4和24.6 pg/ml增至48.8和91.5 pg/ml），但白细胞介素-4的分泌减少（从38.9降至27.9 pg/ml）。相同剂量水平的提取物还刺激了巨噬细胞中的诱导型一氧化氮合酶和溶菌酶，并增强了白细胞介素-6、白细胞介素-1β和肿瘤坏死因子-α的分泌（分别从26.6、73.4和39.6 pg/ml增至60.2、131.0和144.7 pg/ml）。得出的结论是，山药水溶性多糖通过激活免疫细胞和调节免疫相关分子的分泌，对机体具有免疫活性。
