聚合酶链反应和 Goldmann-Witmer 系数在葡萄膜炎中检测 Epstein-Barr 病毒的作用。
Polymerase Chain Reaction and Goldmann-Witmer Coefficient to Examine the Role of Epstein-Barr Virus in Uveitis.
机构信息
a Faculty of Medicine and Health Sciences , Stellenbosch University , Cape Town , South Africa.
b Department of Medical Microbiology , University Medical Center Utrecht , Utrecht , The Netherlands.
出版信息
Ocul Immunol Inflamm. 2019;27(1):108-113. doi: 10.1080/09273948.2017.1370653. Epub 2017 Oct 17.
PURPOSE
To use polymerase chain reaction (PCR) and Goldmann-Witmer Coefficient (GWC) calculation to search for evidence that Epstein-Barr virus (EBV) causes uveitis.
METHODS
A prospective cross-sectional study where participants with positive multiplex EBV PCR results were further investigated by: 1) real-time PCR for EBV viral loads (VL) and 2) EBV GWC.
RESULTS
Eleven of 106 consecutive uveitis patients (10.4%) had positive multiplex PCR for EBV on aqueous humor sampling and 7/11 (63.6%) were HIV-positive. Only 4/10 (40%) cases had detectable intraocular EBV VLs which were always lower than the blood or plasma VL. EBV GWC was negative in all 10 cases tested. In 9/11 (81.8%) of these cases an alternative, more plausible cause of uveitis was identified.
CONCLUSION
We found no evidence of active intraocular replication or antibody production to prove that EBV caused uveitis in these cases. In most cases an alternative treatable cause of uveitis was identified.
目的
利用聚合酶链反应（PCR）和 Goldmann-Witmer 系数（GWC）计算来寻找 Epstein-Barr 病毒（EBV）引起葡萄膜炎的证据。
方法
这是一项前瞻性的病例对照研究，对 multiplex EBV PCR 结果阳性的参与者进行进一步研究：1）实时 EBV 病毒载量（VL）PCR，2）EBV GWC。
结果
106 例连续葡萄膜炎患者中有 11 例（10.4%）在前房水中 multiplex EBV PCR 阳性，其中 7/11（63.6%）为 HIV 阳性。只有 4/10（40%）例可检测到眼内 EBV VL，其始终低于血液或血浆 VL。10 例检测的 EBV GWC 均为阴性。在这些病例中的 9/11（81.8%），发现了另一种更合理的葡萄膜炎病因。
结论
我们没有发现眼内复制或抗体产生的证据来证明 EBV 引起了这些病例的葡萄膜炎。在大多数情况下，发现了另一种可治疗的葡萄膜炎病因。
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