Semina I E, Bazhanova I G, Tsvetkova N V, Remova T N, Ozeretskovskaia M N
Zh Mikrobiol Epidemiol Immunobiol. 1988 Aug(8):76-9.
The physicochemical and biological properties of antigenic complexes isolated from the supernatant fluid of the culture medium of B. pertussis, strains 305 and 475, were studied. The preparations obtained from both strains contained proteins, carbohydrates, nucleic acids and lipids. Electrophoresis in polyacrylamide gel revealed the presence of filamentous hemagglutinin, 4 subunits of B. pertussis toxin and agglutinogens in the antigenic complexes of both strains. The preparations of both strains possessed similar toxic properties and, after their detoxification, produced a pronounced protective effect.
对从百日咳杆菌305株和475株培养基上清液中分离出的抗原复合物的物理化学和生物学特性进行了研究。从这两种菌株获得的制剂都含有蛋白质、碳水化合物、核酸和脂质。聚丙烯酰胺凝胶电泳显示，两种菌株的抗原复合物中都存在丝状血凝素、百日咳毒素的4个亚基和凝集原。两种菌株的制剂具有相似的毒性特性，经解毒后产生明显的保护作用。
