抗体干扰导致心肌肌钙蛋白T定量检测失败。
Cardiac troponin T quantitative assay failure as a result of antibody interference.
作者信息
Fortgens Philip H, Omar Fierdoz
机构信息
Department of Clinical Laboratory Sciences, Division of Chemical Pathology, University of Cape Town, South Africa.
National Health Laboratory Service, Groote Schuur Hospital, South Africa.
出版信息
Afr J Lab Med. 2013 Dec 3;2(1):23. doi: 10.4102/ajlm.v2i1.23. eCollection 2013.
BACKGROUND
Immunoassays are prone to interference by various substances which may cause inaccurate results. This type of interference is difficult to detect analytically.
OBJECTIVE
A case of CARDIAC Troponin T Quantitative reader (Roche Diagnostics) assay failure was detected and investigated in order to ascertain the likely cause.
METHOD
Patient whole blood was mixed with cardiac troponin T-positive blood, patient and control sera were denuded of immunoglobulin G by protein A-affinity chromatography and patient sera were mixed with mouse serum. Samples were analysed on a CARDIAC Troponin T Quantitative reader.
RESULTS
A mixture of patient whole blood and cardiac troponin T-positive blood resulted in assay failure; removal of immunoglobulin G from patient sera reversed the cardiac troponin T assay failure; the addition of mouse serum as a heterophile antibody blocking agent had no effect.
CONCLUSION
It is proposed that the interference resulting in assay failure may not be because of a heterophile antibody, but rather a result of a circulating autoantibody to cardiac troponin T, which may compete with antibody assay reagents for binding sites.
背景
免疫测定容易受到各种物质的干扰，这可能会导致结果不准确。这种干扰在分析上很难检测到。
目的
检测并调查一例心肌肌钙蛋白T定量分析仪（罗氏诊断公司）检测失败的病例，以确定可能的原因。
方法
将患者全血与心肌肌钙蛋白T阳性血液混合，通过蛋白A亲和色谱法去除患者和对照血清中的免疫球蛋白G，并将患者血清与小鼠血清混合。在心肌肌钙蛋白T定量分析仪上对样本进行分析。
结果
患者全血与心肌肌钙蛋白T阳性血液的混合物导致检测失败；从患者血清中去除免疫球蛋白G可逆转心肌肌钙蛋白T检测失败；添加小鼠血清作为嗜异性抗体阻断剂没有效果。
结论
有人提出，导致检测失败的干扰可能不是由于嗜异性抗体，而是由于循环中的心肌肌钙蛋白T自身抗体，它可能与抗体检测试剂竞争结合位点。
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