载紫杉醇硒纳米粒诱导癌细胞凋亡的高效性。
Efficient induction of apoptosis in cancer cells by paclitaxel-loaded selenium nanoparticles.
机构信息
Department of Biosciences & Bioengineering, Indian Institute of Technology, Guwahati, Assam, India.
Centre for Nanotechnology, Indian Institute of Technology Guwahati, Guwahati-39, Assam, India.
出版信息
Nanomedicine (Lond). 2017 Nov;12(21):2641-2651. doi: 10.2217/nnm-2017-0189. Epub 2017 Oct 18.
AIM
To develop selenium nanoparticles (SeNPs)-based delivery systems for paclitaxel (PTX) and assess their antiproliferative efficacy against cancer cells in vitro with potential mechanistic insight.
METHODS
Pluronic F-127 stabilized SeNPs were prepared and characterized. Effects of PTX-loaded SeNPs on lung (A549), breast (MCF7), cervical (HeLa) and colon (HT29) cancer cells were studied by viability assay complemented with flow-cytometric analyses of cell cycle, apoptosis, mitochondrial membrane potential, intracellular reactive oxygen species and caspase activity.
RESULTS
PTX-loaded SeNPs demonstrated significant antiproliferative activity against cancer cells. Cell cycle analyses of PTX-SeNPs treated cells established G/M phase arrest in a dose-dependent manner leading to apoptosis. Further investigation revealed disruption of mitochondrial membrane potential orchestrated with induction of reactive oxygen species leading to the activation of caspases, key players of apoptotic cell death.
CONCLUSION
Efficient induction of apoptosis in various cancer cells by PTX-loaded SeNPs, with appropriate future studies, might lead to potential anticancer strategies.
目的
开发基于硒纳米粒子（SeNPs）的紫杉醇（PTX）递送系统，并评估其在体外对癌细胞的抗增殖功效，同时深入了解潜在的作用机制。
方法
制备并表征了泊洛沙姆 F-127 稳定的 SeNPs。通过细胞活力测定法，结合细胞周期、凋亡、线粒体膜电位、细胞内活性氧和半胱天冬酶活性的流式细胞分析，研究负载 PTX 的 SeNPs 对肺癌（A549）、乳腺癌（MCF7）、宫颈癌（HeLa）和结肠癌（HT29）癌细胞的影响。
结果
负载 PTX 的 SeNPs 对癌细胞表现出显著的抗增殖活性。PTX-SeNPs 处理细胞的细胞周期分析显示，G/M 期阻滞呈剂量依赖性，导致细胞凋亡。进一步的研究表明，线粒体膜电位的破坏与活性氧的诱导有关，进而激活了半胱天冬酶，这是细胞凋亡的关键执行者。
结论
负载 PTX 的 SeNPs 能够有效诱导各种癌细胞凋亡，未来适当的研究可能会为潜在的抗癌策略提供依据。
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