Anisimová E, Nováková Z, Roubal J
Department of Experimental Virology, Institute of Sera and Vaccines, Prague, Czechoslovakia.
Acta Virol. 1988 Sep;32(5):417-25.
Morphological changes were induced by cis-dichlorodiammine platinum (II) (cis-DDP) in two Epstein-Barr virus (EBV) transformed cell lines: the productive P3HR-1 and the nonproductive Raji cell line. In P3HR-1 cells cis-DDP induced synthesis of viral antigens, viral particles and morphological changes characteristic for virus replication. In Raji cells, the virus replicative cycle was not induced and virus-specific morphological changes were limited to the sporadic appereance of some very early alterations in cell morphology. However, in Raji cells, but not in P3HR-1 cells, up to 20 per cent of the cell population exhibited differentiation-related changes towards plasma cell morphology. The most advances stage of cell differentiation detected was classified as plasmablast. Cis-DDP also induced some changes associated with the cytostatic effect of the drug. In the treated cell population cytokinesis was inhibited and frequently multinuclear cells appeared; moreover, extensive degenerative changes were observed.
顺二氯二氨铂（II）（顺铂）在两种爱泼斯坦-巴尔病毒（EBV）转化细胞系中诱导了形态学变化：有生产性的P3HR-1细胞系和无生产性的Raji细胞系。在P3HR-1细胞中，顺铂诱导病毒抗原、病毒颗粒的合成以及病毒复制特有的形态学变化。在Raji细胞中，未诱导病毒复制周期，病毒特异性形态学变化仅限于细胞形态中一些非常早期改变的零星出现。然而，在Raji细胞而非P3HR-1细胞中，高达20%的细胞群体表现出向浆细胞形态的分化相关变化。检测到的细胞分化最晚期阶段被归类为成浆细胞。顺铂还诱导了一些与该药物细胞抑制作用相关的变化。在处理后的细胞群体中，胞质分裂受到抑制，多核细胞频繁出现；此外，还观察到广泛的退行性变化。
