Department of Surgery, Anesthesiology, and Radiology, Faculty of Veterinary Medicine, Cairo University, Giza, 12211, Egypt.
Department of Pathology, Faculty of Veterinary Medicine, Cairo University, Giza, 12211, Egypt.
Stem Cell Res Ther. 2017 Oct 18;8(1):235. doi: 10.1186/s13287-017-0682-3.
Gastric mucosal defect could result from several causative factors including the use of nonsteroidal anti-inflammatory drugs, Helicobacter pylori infection, gastrointestinal and spinal cord diseases, and neoplasia. This study was performed to achieve a novel simple, inexpensive, and effective surgical technique for the repair of gastric mucosal defect.
Six adult male mongrel dogs were divided into two groups (three dogs each). In the control positive group (C + ve), dogs were subjected to surgical induction of gastric mucosal defect and then treated using traditional medicinal treatment for such a condition. In the amniotic membrane (AM) group, dogs were subjected to the same operation and then fresh AM allograft was applied. Clinical, endoscopic, biochemical (serum protein and lipid and pepsin activity in gastric juice), histopathological, and immunohistochemistry evaluations were performed.
Regarding endoscopic examination, there was no sign of inflammatory reaction around the grafted area in the AM group compared to the C + ve group. The leukocytic infiltration in the gastric ulcer was well detected in the control group and was less observed in the AM group. In the AM group, the concentrations of both protein and lipid profiles were nearly the same as those in serum samples taken preoperatively at zero time, which indicated that the AM grafting acted the same as gastric mucosa. The re-epithelization of the gastric ulcer in the C + ve group was not yet detected at 21 days, while in the AM group it was well observed covering most of the gastric ulcer. AM accelerated the re-epithelization of the gastric ulcer. The fibrous connective tissue and the precursor of collagen (COL IA1) were poorly detected in the gastric ulcer with AM application.
Using fresh AM allograft for repairing gastric mucosal defect in dogs showed great impact as a novel method to achieve optimum reconstruction of the gastric mucosal architecture and restoration of pre-epithelial, epithelial, and post-epithelial normal gastric mucosal barriers.
胃黏膜缺损可由多种致病因素引起，包括非甾体抗炎药、幽门螺杆菌感染、胃肠道和脊髓疾病以及肿瘤。本研究旨在探索一种新的简单、廉价、有效的胃黏膜缺损修复手术技术。
将 6 只成年雄性杂种狗分为两组（每组 3 只）。在对照阳性组（C+ve）中，狗接受胃黏膜缺损的手术诱导，然后用传统药物治疗这种情况。在羊膜（AM）组中，狗接受相同的手术，然后应用新鲜 AM 同种异体移植物。进行临床、内镜、生化（血清蛋白和脂质及胃液胃蛋白酶活性）、组织病理学和免疫组织化学评估。
关于内镜检查，与 C+ve 组相比，AM 组移植物区域周围没有炎症反应的迹象。在对照组中，胃溃疡中的白细胞浸润得到很好的检测，而在 AM 组中则较少观察到。在 AM 组中，蛋白质和脂质谱的浓度与术前零时取自血清样本的浓度几乎相同，这表明 AM 移植物的作用与胃黏膜相同。在 C+ve 组，胃溃疡的再上皮化在 21 天时尚未检测到，而在 AM 组中，它已被很好地观察到，覆盖了大部分胃溃疡。AM 加速了胃溃疡的再上皮化。在 AM 应用中，胃溃疡中的纤维结缔组织和胶原（COL IA1）前体的检测较差。
在狗中应用新鲜 AM 同种异体移植物修复胃黏膜缺损显示出巨大的影响，作为一种新的方法，可以实现胃黏膜结构的最佳重建，并恢复上皮前、上皮和上皮后正常胃黏膜屏障。
