Duarte Ian Göedert Leite, Duval-Araujo Ivana
Medical School, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil.
Department of Surgery, Medical School, UFMG, Belo Horizonte, MG, Brazil.
Acta Cir Bras. 2014 May;29(5):334-9. doi: 10.1590/s0102-86502014000500008.
To investigate amniotic membrane as a biological dressing in infected wound healing in rabbits.
The use of preserved amniotic membranes (AMs) was examined using 15 rabbits with experimentally induced wound infections on their backs. Healing was histologically evaluated during different phases including inflammation, granulation, epithelialization, and fibroplasia. The animals were distributed into three groups for histological study at seven, 14, 21, and 28 days post-wound induction. Group A did not receive treatment: the wound was left exposed and dry; Group B received a daily exposure treatment with collagenase; and Group C received one AM, which also remained exposed.
A marked reduction of the inflammatory phase was observed in Group C at 21 days, and the granulation phase of this healing increased at 14 days. Epithelialization was similar among the three groups, and fibroplasia was more pronounced in Group C at 14 days. Furthermore, gradual collagen organization also began for the animals in Group B at 14 days.
The amniotic membrane did not significantly alter the inflammation, epithelialization, or fibroplasia phases but did increase angiogenesis up to Day 14 compared with the dry dressing and collagenase treatments.
研究羊膜作为生物敷料在兔感染伤口愈合中的作用。
使用15只背部经实验诱导产生伤口感染的兔子，对保存的羊膜（AM）的使用情况进行研究。在炎症、肉芽形成、上皮化和成纤维细胞增生等不同阶段对愈合情况进行组织学评估。在伤口诱导后的第7、14、21和28天，将动物分为三组进行组织学研究。A组不接受治疗：伤口保持暴露和干燥；B组每天用胶原酶进行暴露治疗；C组接受一片羊膜，同样保持暴露。
C组在第21天时炎症期明显缩短，其愈合的肉芽形成期在第14天时增加。三组的上皮化情况相似，C组在第14天时成纤维细胞增生更为明显。此外，B组动物在第14天时也开始逐渐出现胶原组织形成。
与干敷料和胶原酶治疗相比，羊膜在第14天之前并未显著改变炎症、上皮化或成纤维细胞增生阶段，但确实增加了血管生成。
