基于聚集诱导发光型荧光探针对 l-乳酸的灵敏和特异检测。
Sensitive and Specific Detection of l-Lactate Using an AIE-Active Fluorophore.
机构信息
Department of Chemical and Biological Engineering, The Hong Kong University of Science and Technology , Clear Water Bay, Kowloon, Hong Kong, China.
Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction, Department of Chemistry, The Hong Kong University of Science and Technology , Clear Water Bay, Kowloon, Hong Kong, China.
出版信息
ACS Appl Mater Interfaces. 2017 Nov 8;9(44):38153-38158. doi: 10.1021/acsami.7b10178. Epub 2017 Oct 24.
l-Lactate is a vital biomarker for many diseases and physiological fatigue. An AIE-active fluorophore (TPE-HPro) is combined with l-lactate oxidase (LOx) to determine l-lactate in aqueous fluid. The assay shows excellent sensitivity and anti-interference performance with a limit of detection (LOD) of 5.5 μM. In addition, sensitive detection of l-lactate is achieved even in a protein-rich environment. It is proposed that quantification of l-lactate be performed at 20 or 60 min in the current method. These characteristics endow the fluorometric assay with great potential for biomedical diagnostics.
l-乳酸是许多疾病和生理疲劳的重要生物标志物。将 AIE 活性荧光团（TPE-HPro）与 l-乳酸氧化酶（LOx）结合，可在水溶液中测定 l-乳酸。该测定法具有出色的灵敏度和抗干扰性能，检测限（LOD）为 5.5 μM。此外，即使在富含蛋白质的环境中，也能实现对 l-乳酸的灵敏检测。建议在当前方法中分别在 20 分钟或 60 分钟时进行 l-乳酸的定量。这些特性使荧光测定法在生物医学诊断中具有巨大的潜力。
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