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在无血清培养基中培养的人鼻甲来源间充质干细胞特性评估。

Evaluation of characteristic of human turbinate derived mesenchymal stem cells cultured in the serum free media.

作者信息

Hwang Se Hwan, Lee WeonSun, Park Sang Hi, Lee Hee Jin, Park Sun Hwa, Lee Dong Chang, Lim Mi Hyun, Back Sang A, Yun Byeong Gon, Jeun Jung Ho, Lim Jung Yeon, Kang Jun Myung, Kim Sung Won

机构信息

Department of Otolaryngology-Head and Neck Surgery, College of Medicine, The Catholic University of Korea, Seoul, Korea.

Institute of Clinical Medicine Research, College of Medicine, Catholic University of Korea, Seoul, Korea.

出版信息

PLoS One. 2017 Oct 19;12(10):e0186249. doi: 10.1371/journal.pone.0186249. eCollection 2017.

DOI:10.1371/journal.pone.0186249
PMID:29049314
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5648157/
Abstract

We evaluated the effect of serum-free and xeno-cultivation (SFXFM) on the characterization, proliferation, and differentiation properties of human nasal stem cells (airway tissue; hTMSCs). hTMSCs were isolated from 10 patients, after which patient samples were separated into two groups, an SFXFM group and a control group. The control group was treated with bovine serum-containing medium. FACS analysis revealed that SFXFM-cultured hTMSCs maintained a characteristic mesenchymal stem cell phenotype. hTMSC proliferation was not influenced by SFXFM. In addition, upregulation of IL-8 and GM-CSF and downregulation of RANTES expression were shown in response to SFXFM. Moreover, two-lineage differentiation properties (osteocyte and adipocyte) of hTMSCs were enhanced under SFXFM. Finally, the genetic stability of SFXFM-cultured hTMSCs was demonstrated by normal karyotype results. SFXFM enables good expansion, multipotentiality, and normal genotype maintenance of MSCs. Moreover, this approach serves as a substitute to conventional media for the cultivation of capable MSCs for upcoming medical applications.

摘要

我们评估了无血清异种培养（SFXFM）对人鼻干细胞（气道组织；hTMSCs）的特性、增殖及分化特性的影响。从10名患者中分离出hTMSCs，之后将患者样本分为两组，即SFXFM组和对照组。对照组用含牛血清的培养基处理。流式细胞术分析显示，经SFXFM培养的hTMSCs维持了典型的间充质干细胞表型。hTMSC的增殖不受SFXFM影响。此外，SFXFM可使IL-8和GM-CSF上调，RANTES表达下调。而且，在SFXFM条件下hTMSCs的双系分化特性（成骨细胞和脂肪细胞）增强。最后，正常核型结果证明了经SFXFM培养的hTMSCs的遗传稳定性。SFXFM能实现间充质干细胞的良好扩增、多能性及正常基因型维持。此外，这种方法可替代传统培养基用于培养有能力的间充质干细胞以用于未来的医学应用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5003/5648157/c36c947f1bb8/pone.0186249.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5003/5648157/cdfa422361b5/pone.0186249.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5003/5648157/d39d816892eb/pone.0186249.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5003/5648157/6e2d2f05eb76/pone.0186249.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5003/5648157/a085c04d4a2b/pone.0186249.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5003/5648157/2431db6f7175/pone.0186249.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5003/5648157/6e71fae2b10c/pone.0186249.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5003/5648157/c36c947f1bb8/pone.0186249.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5003/5648157/cdfa422361b5/pone.0186249.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5003/5648157/d39d816892eb/pone.0186249.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5003/5648157/6e2d2f05eb76/pone.0186249.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5003/5648157/a085c04d4a2b/pone.0186249.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5003/5648157/2431db6f7175/pone.0186249.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5003/5648157/6e71fae2b10c/pone.0186249.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5003/5648157/c36c947f1bb8/pone.0186249.g007.jpg

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