Tang Lingyi, Pan Wu, Zhu Guisong, Liu Zhihui, Lv Dongling, Jiang Meng
Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210029, PR China.
Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210029, PR China.
Oncotarget. 2017 Jul 15;8(41):69768-69778. doi: 10.18632/oncotarget.19264. eCollection 2017 Sep 19.
Angiogenesis is a process of new blood vessel formation from pre-existing vessels. It is a normal and vital process in growth and development, as well as in wound healing and in the formation of granulation tissue. Total flavones of (TFA) are the major constituents of the traditional Chinese herb L. Medic. The aim of this study is to investigate the effect of TFA on angiogenic ability using human umbilical vein endothelial cells (HUVECs) and chick chorioallantoic membrane (CAM) . HUVECs were treated with TFA at different concentrations. Cell viability, cell cycle progression, cell apoptosis, cell migration and tubular formation were investigated. The expression of vascular endothelial growth factor (VEGF) and kinase insert domain receptor (KDR, VEGFR-2) was examined by immunohistochemistry to identify mechanism of action of TFA. CAM model was used to evaluate the effect of TFA on angiogenesis . Our results showed that TFA promoted HUVECs proliferation in a dose- and time-dependent manner. It increased HUVECs migratory ability and the number of tubular structure, promoted vessel formation in HUVECs culture and CAM model. Furthermore, TFA treatment resulted in a decrease in cell apoptosis and enhanced the expression of VEGF and KDR. Taken together, TFA, as the major active component isolated from the traditional Chinese herb L. Medic, could enhance angiogenic ability of HUVECs and CAM . TFA may be used in the treatment of wound healing and ischemic/reperfusion injuries.
血管生成是一个从已有的血管形成新血管的过程。它是生长发育、伤口愈合以及肉芽组织形成过程中正常且至关重要的过程。[此处原文缺失植物名称]总黄酮（TFA）是传统中药[此处原文缺失植物名称]的主要成分。本研究的目的是使用人脐静脉内皮细胞（HUVECs）和鸡胚绒毛尿囊膜（CAM）来研究TFA对血管生成能力的影响。用不同浓度的TFA处理HUVECs。研究细胞活力、细胞周期进程、细胞凋亡、细胞迁移和管状结构形成。通过免疫组织化学检测血管内皮生长因子（VEGF）和激酶插入结构域受体（KDR，VEGFR - 2）的表达，以确定TFA的作用机制。使用CAM模型评估TFA对血管生成的影响。我们的结果表明，TFA以剂量和时间依赖性方式促进HUVECs增殖。它增加了HUVECs的迁移能力和管状结构的数量，促进了HUVECs培养物和CAM模型中的血管形成。此外，TFA处理导致细胞凋亡减少，并增强了VEGF和KDR的表达。综上所述，TFA作为从传统中药[此处原文缺失植物名称]中分离出的主要活性成分，可以增强HUVECs和CAM的血管生成能力。TFA可用于治疗伤口愈合和缺血/再灌注损伤。
