Department of Chemical and Biological Engineering, Korea University, Seoul 02841, South Korea.
Department of Chemical and Biological Engineering, Korea University, Seoul 02841, South Korea.
Biosens Bioelectron. 2018 Mar 15;101:96-102. doi: 10.1016/j.bios.2017.10.018. Epub 2017 Oct 11.
Alzheimer's disease (AD) is a neurodegenerative disease associated with the loss of nerve cells in the brain. The disease is affected by multifactorial pathways and leads to changes in related biomolecular levels as AD progresses. Therefore, AD should be diagnosed with combined detection of several lesions to improve accuracy. Amyloid beta 1-40, 1-42 and τ (tau) protein are milestones in AD pathology and can be used as main screening and diagnostic target markers. Here, we suggest a highly selective biosensor for detection of AD core biomarkers on one platform through distinct localized surface plasmon resonance (LSPR) depending on gold nanoparticles shapes, called a shape-code biosensor. This plasmonic sensor consists of only gold nanoparticles and antibody, but does not need additory methods for precise separation from multifarious samples and identification of markers. Under physiological condition, we determined a detection limit of 34.9fM for amyloid beta (Aβ) 1-40, 26fM for Aβ 1-42 and 23.6fM for τ protein corresponding to the ~ 1, ~ 2.23 and ~ 3.12nm of Rayleigh scattering peak shift on shape-code plasmon system for each biomarker in mimicked blood. This is the first highly sensitive shape-code biosensor to detect AD biomarkers which can be used to diagnose AD easily in the future.
阿尔茨海默病（AD）是一种与大脑神经细胞丧失有关的神经退行性疾病。该疾病受多因素途径影响，并随着 AD 的进展导致相关生物分子水平的变化。因此，AD 应该通过几种病变的联合检测来诊断，以提高准确性。β淀粉样蛋白 1-40、1-42 和 τ（tau）蛋白是 AD 病理学的里程碑，可作为主要的筛选和诊断靶标标志物。在这里，我们通过独特的局域表面等离子体共振（LSPR）建议在一个平台上对 AD 核心生物标志物进行高选择性检测的生物传感器，称为形状码生物传感器。这种等离子体传感器仅由金纳米粒子和抗体组成，但不需要额外的方法从多种样品中进行精确分离和标记物识别。在生理条件下，我们确定了对模拟血液中每种生物标志物的1、2.23 和~3.12nm 的瑞利散射峰位移，对β淀粉样蛋白（Aβ）1-40 的检测限为 34.9fM，对 Aβ1-42 的检测限为 26fM，对τ蛋白的检测限为 23.6fM。这是第一个用于检测 AD 生物标志物的高灵敏度形状码生物传感器，将来可以用于 AD 的诊断。
