Wang Mengyue, Wan Huiyu, Wang Yanjiao, Yuan Hui, Ni Qingbin, Sun Baoliang, Sun Jingyi, Wang Ying
School of Traditional Chinese Medicine, Shandong University of Traditional Chinese Medicine, Jinan, 250355, Shandong, China.
Shandong First Medical University & Shandong Academy of Medical Sciences, The Second Affiliated Hospital, Taian, 271000, Shandong, China.
Transl Stroke Res. 2025 Apr;16(2):217-226. doi: 10.1007/s12975-023-01204-x. Epub 2023 Nov 21.
Sensitive and accurate methods for early detection of acute ischemic stroke (AIS) are essential for timely treatment and prognostic assessment of patients. In this study, we report a microfluidics-based ultrasensitive surface-enhanced Raman scattering (SERS) immunoassay device for the quantitative determination of multiplex biomarkers in AIS. By preparing 5,5'-dithiobis-2-nitrobenzoic acid (DTNB) antibody-modified gold nanoparticles (AuNPs) on SERS devices as SERS probes, the biomarkers in whole blood of AIS were accurately captured and further visualized for SERS signal intensity quantitative analysis of six biomarkers in the blood samples. It is worth mentioning that the limit of detection (LOD) of the method can reach the level of fg/mL, with excellent sensitivity and selectivity. Meanwhile, the analytical comparison with ELISA method showed that the detection results of both methods were consistent, which verified the feasibility of the assembled device. The SERS immunoassay device detection provides a powerful strategy for the prediction, early diagnosis and dynamic monitoring of prognosis of AIS with a wide range of clinical practice prospects.
用于急性缺血性中风（AIS）早期检测的灵敏且准确的方法对于患者的及时治疗和预后评估至关重要。在本研究中，我们报告了一种基于微流控的超灵敏表面增强拉曼散射（SERS）免疫分析装置，用于定量测定AIS中的多重生物标志物。通过在SERS装置上制备5,5'-二硫代双-2-硝基苯甲酸（DTNB）抗体修饰的金纳米颗粒（AuNPs）作为SERS探针，准确捕获AIS全血中的生物标志物，并进一步可视化以对血样中的六种生物标志物进行SERS信号强度定量分析。值得一提的是，该方法的检测限（LOD）可达到fg/mL水平，具有出色的灵敏度和选择性。同时，与ELISA方法的分析比较表明，两种方法的检测结果一致，这验证了组装装置的可行性。SERS免疫分析装置检测为AIS的预测、早期诊断和预后动态监测提供了有力策略，具有广泛的临床应用前景。
