State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Materials Science and Engineering, Donghua University, Shanghai 201620, China.
College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, China.
Colloids Surf B Biointerfaces. 2018 Jan 1;161:94-102. doi: 10.1016/j.colsurfb.2017.10.041. Epub 2017 Oct 14.
Surgical adhesive is the optimal candidate for the replacement of traditional mechanical wound closure. In our paper, mussel adhesive proteins inspired hydrogel adhesive was prepared with 3, 4-dihydroxyphyenylanine acrylamide (DOPA-AA), poly (ethylene glycol) diacrylate (PEGDAA) and thiolated chitosan (CSS) by UV irradiation. DOPA-AA, containing catechol group and vinyl group, was successful synthesized and characterized by FTIR and HNMR. The gelation time, equilibrium water content, degradation, materials properties and adhesive strength of the hydrogels were studied. We found that the gelation time was retarded and the materials mechanical strength was decreased because of the inhibitory effect of catechol group. Equilibrium water content was slightly affect by the increasing concentration of DOPA-AA (1-5%). Nevertheless, catechol group can remarkably improve the adhesive properties because of the complex and durable interactions of the hydrogel, especially, the interaction between the thiol group of CSS and catechol group of DOPA-AA, which also greatly slowed down the degradation of the adhesive hydrogels. CSS and DOPA-AA was introduced to ensure the adhesive properties, DOPA-AA lend the adhesive nature to hydrogel and CSS can protect the catechol group from oxidation and enhance durable adhesion. Moreover, cytotoxicity of the resulting hydrogels showed that the L929 cell viability was weaken, it mostly probably induced by the catechol oxidation.
外科用黏合剂是替代传统机械伤口闭合的最佳选择。在我们的论文中，通过紫外光照射，使用含有邻苯二酚基团和乙烯基的 3,4-二羟基苯丙氨酸丙烯酰胺(DOPA-AA)、聚乙二醇二丙烯酸酯(PEGDAA)和巯基化壳聚糖(CSS)制备了贻贝类黏附蛋白启发的水凝胶黏合剂。成功合成并通过傅里叶变换红外光谱(FTIR)和核磁共振氢谱(HNMR)对 DOPA-AA 进行了表征。研究了水凝胶的胶凝时间、平衡含水量、降解、材料性能和黏附强度。我们发现，由于邻苯二酚基团的抑制作用，凝胶时间延长，材料力学强度降低。平衡含水量受 DOPA-AA 浓度(1-5%)的增加影响较小。然而，邻苯二酚基团可以显著提高黏附性能，因为水凝胶的复杂且持久的相互作用，特别是 CSS 的巯基与 DOPA-AA 的邻苯二酚基团之间的相互作用，这也大大减缓了黏附水凝胶的降解。引入 CSS 和 DOPA-AA 是为了确保黏附性能，DOPA-AA 赋予水凝胶黏附性，CSS 可以保护邻苯二酚基团免受氧化并增强持久的黏附性。此外，所得水凝胶的细胞毒性表明 L929 细胞活力减弱，这很可能是由邻苯二酚氧化引起的。
