Arbovirology division, Regional Medical Research Centre, NE Region, ICMR, Dibrugarh, 786001, Assam, India.
Sci Rep. 2017 Oct 20;7(1):13716. doi: 10.1038/s41598-017-14091-8.
Japanese encephalitis (JE) is a major contributor for viral encephalitis in Asia. Vaccination programme has limited success for largely populated JE endemic countries like India and disease exposure is unavoidable. Involvement of chemokines and its co-receptors for adverse prognosis of JE have been documented both in vitro and in vivo. Identification of the genetic predisposing factor for JE infection in humans is crucial but not yet established. Therefore, we investigated the association of single nucleotide polymorphisms (SNPs) in chemokines (CCL2 and CCL5) and its co-receptors (CCR2 and CCR5) with their protein level for JE. The study enrolled 87 symptomatic JE cases (mild: severe = 24:63) and 94 asymptomatic controls. Our study demonstrated that CCL2 (rs1024611G), CCL5 (rs2280788G) and CCR2 (rs1799864A) significantly associated with JE (Odds ratio = 1.63, 2.95 and 2.62, respectively and P = 0.045, P = 0.05 and P = 0.0006, respectively). The study revealed that rs1024611G allele was associated with elevated level of CCL2. CCL5 elevation associated with JE mortality having a Cox proportional hazard of 1.004 (P = 0.033). In conclusion, SNPs of chemokine viz. CCL2 (rs1024611G) and its receptor CCR2 (rs1799864A) significantly associated with JE which may serve as possible genetic predisposing factor and CCL5 protein level may act as marker for disease survival.
日本脑炎（JE）是亚洲病毒性脑炎的主要病因。对于印度等人口众多的 JE 流行国家，疫苗接种计划的效果有限，而且疾病暴露是不可避免的。趋化因子及其共受体在 JE 的不良预后中的作用在体外和体内都有记录。确定人类 JE 感染的遗传易感性因素至关重要，但尚未确定。因此，我们研究了趋化因子（CCL2 和 CCL5）及其共受体（CCR2 和 CCR5）中的单核苷酸多态性（SNPs）与 JE 时的蛋白水平的关系。该研究纳入了 87 例有症状的 JE 病例（轻度：重度=24:63）和 94 例无症状对照。我们的研究表明，CCL2（rs1024611G）、CCL5（rs2280788G）和 CCR2（rs1799864A）与 JE 显著相关（比值比分别为 1.63、2.95 和 2.62，P 值分别为 0.045、0.05 和 0.0006）。研究表明，rs1024611G 等位基因与 CCL2 水平升高相关。与 JE 死亡率相关的 CCL5 升高，Cox 比例风险比为 1.004（P=0.033）。结论：趋化因子 SNP，即 CCL2（rs1024611G）和其受体 CCR2（rs1799864A）与 JE 显著相关，这可能是潜在的遗传易感性因素，而 CCL5 蛋白水平可能是疾病生存的标志物。
