microRNA-937 通过下调 FOXL2 抑制胃癌细胞的增殖和转移。
MicroRNA-937 inhibits cell proliferation and metastasis in gastric cancer cells by downregulating FOXL2.
出版信息
Cancer Biomark. 2017 Dec 12;21(1):105-116. doi: 10.3233/CBM-170310.
BACKGROUND
Despite advancement in the treatment, the mortality of gastric cancer remains high. MicroRNAs (miRNAs) are involved in tumor development and progression by regulating signaling pathways. In this study, we investigated the role of miR-937 in gastric cancer cells.
METHODS
MiR-937 expression was determined in gastric cancer and matched normal tissues, and human gastric cancer cell lines by quantitative real-time PCR (qRT-PCR). Effects of miR-937 on cell proliferation, migration and invasion were analyzed by MTT and Transwell assays, respectively. In addition, the regulation of FOXL2 by miR-937 was evaluated by western blotting and luciferase reporter assays. Effects of FOXL2 on cell proliferation, migration and invasion were detected again.
RESULTS
MiR-937 level was low expressed in gastric cancer tissues and cell lines. Overexpression of miR-937 inhibited cell viability, colony formation and promoted apoptosis in gastric cancer cells. MiR-937 overexpression also inhibited cell migration and invasion by inhibiting the EMT process. Furthermore, we found that FOXL2 was a direct target of miR-937 and negatively regulated by miR-937. Moreover, miR-937 overexpression inhibited the activation of the PI3K/AKT pathway. Furthermore, we found that overexpression of FOXL2 promoted cell viability, colony formation, migration, invasion, and inhibited apoptosis in gastric cancer cells.
CONCLUSIONS
MiR-937 inhibits the proliferation and metastasis of gastric cancer cells by targeting FOXL2 via inactivation of PI3K/AKT signaling pathway. These results suggest that miR-937 may be a potential target for the treatment of gastric cancer.
背景
尽管治疗方法有所进步，但胃癌的死亡率仍然很高。microRNAs（miRNAs）通过调节信号通路参与肿瘤的发生和发展。在本研究中，我们研究了 miR-937 在胃癌细胞中的作用。
方法
通过定量实时 PCR（qRT-PCR）检测胃癌及配对正常组织和人胃癌细胞系中 miR-937 的表达。通过 MTT 和 Transwell 分析分别检测 miR-937 对细胞增殖、迁移和侵袭的影响。此外，通过 Western blot 和荧光素酶报告基因实验评估 miR-937 对 FOXL2 的调控作用。再次检测 FOXL2 对细胞增殖、迁移和侵袭的影响。
结果
miR-937 在胃癌组织和细胞系中低表达。miR-937 的过表达抑制胃癌细胞的活力、集落形成并促进细胞凋亡。miR-937 过表达还通过抑制 EMT 过程抑制细胞迁移和侵袭。此外，我们发现 FOXL2 是 miR-937 的直接靶标，并受 miR-937 负调控。此外，miR-937 过表达抑制 PI3K/AKT 信号通路的激活。此外，我们发现过表达 FOXL2 促进胃癌细胞的活力、集落形成、迁移、侵袭，并抑制细胞凋亡。
结论
miR-937 通过靶向 FOXL2 抑制 PI3K/AKT 信号通路的激活来抑制胃癌细胞的增殖和转移。这些结果表明 miR-937 可能是治疗胃癌的潜在靶点。
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