Department of Microbiology, University of Manitoba, Winnipeg, Canada.
National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Canada.
Antimicrob Agents Chemother. 2017 Dec 21;62(1). doi: 10.1128/AAC.01514-17. Print 2018 Jan.
is a notorious opportunistic pathogen that is prevalent mainly in hospital settings. The ability of to adapt and to survive in a range of environments has been a key factor for its persistence and success as an opportunistic pathogen. In this study, we investigated the effect of temperature on the clinically relevant phenotypes displayed by at 37°C and 28°C. Surface-associated motility was significantly reduced at 28°C, while biofilm formation on plastic surfaces was increased at 28°C. Decreased susceptibility to aztreonam and increased susceptibility to trimethoprim-sulfamethoxazole were observed at 28°C. No differences in virulence, as assayed in a model, were observed. Proteomic analysis showed differential expression of 629 proteins, of which 366 were upregulated and 263 were downregulated at 28°C. Upregulation of the Csu and iron uptake proteins at 28°C was a key finding for understanding some of the phenotypes displayed by at 28°C.
是一种臭名昭著的机会致病菌，主要在医院环境中流行。能够适应和在多种环境中生存，是其作为机会致病菌持续存在和成功的关键因素。在这项研究中，我们研究了温度对 在 37°C 和 28°C 下表现出的临床相关表型的影响。在 28°C 时，表面相关的迁移能力显著降低，而在 28°C 时，在塑料表面形成生物膜的能力增加。在 28°C 时，对氨曲南的敏感性降低，对甲氧苄啶-磺胺甲恶唑的敏感性增加。在 模型中，未观察到毒力的差异。蛋白质组学分析显示，在 28°C 时，有 629 种蛋白质的表达差异，其中 366 种上调，263 种下调。在 28°C 时，Csu 和铁摄取蛋白的上调是理解 在 28°C 时表现出的一些表型的关键发现。
