Department of Medical Sciences, College of Medical Sciences, Soonchunhyang University, Asan 31538, Republic of Korea.
Department of Biomedical Laboratory Science, College of Medical Sciences, Soonchunhyang University, Asan 31538, Republic of Korea.
Microbiology (Reading). 2020 Aug;166(8):717-726. doi: 10.1099/mic.0.000930.
is a multidrug-resistant opportunistic pathogen that affects patients with a compromised immune system and is becoming increasingly important as a hospital-derived infection. This pathogen is difficult to treat owing to its intrinsic multidrug resistance and ability to form antimicrobial-tolerant biofilms. In the present study, we aimed to assess the potential use of zerumbone as a novel anti-biofilm and/or anti-virulence agent against . The results showed that zerumbone at sub-inhibitory doses decreased biofilm formation and disrupted established biofilms. The zerumbone-induced decrease in biofilm formation was dose-dependent based on the results of microtitre plate biofilm assays and confocal laser scanning microscopy. In addition, our data validated the anti-virulence efficacy of zerumbone, wherein it significantly interfered with the motility of . To support these phenotypic results, transcriptional analysis revealed that zerumbone downregulated the expression of biofilm- and virulence-associated genes (, , and ) in . Overall, our findings suggested that zerumbone might be a promising bioactive agent for the treatment of biofilm- and virulence-related infections caused by multidrug-resistant .
洋葱伯克霍尔德菌是一种具有多重耐药性的机会性病原体，影响免疫系统受损的患者，并且作为医院获得性感染变得越来越重要。由于其内在的多重耐药性和形成抗微生物药物耐受生物膜的能力，这种病原体难以治疗。在本研究中，我们旨在评估姜烯酮作为一种新型抗生物膜和/或抗毒力剂治疗洋葱伯克霍尔德菌的潜力。结果表明，亚抑制剂量的姜烯酮可减少生物膜形成并破坏已建立的生物膜。微量滴定板生物膜测定和共聚焦激光扫描显微镜的结果表明，姜烯酮诱导的生物膜形成减少呈剂量依赖性。此外，我们的数据验证了姜烯酮的抗毒力功效，其中它显著干扰了洋葱伯克霍尔德菌的运动性。为了支持这些表型结果，转录分析表明，姜烯酮下调了生物膜和毒力相关基因（、、和）在洋葱伯克霍尔德菌中的表达。总的来说，我们的研究结果表明，姜烯酮可能是一种有前途的生物活性药物，可用于治疗由多重耐药性洋葱伯克霍尔德菌引起的生物膜和毒力相关感染。
