State Key Laboratory of Breeding Base for Zhejiang Sustainable Pest and Disease Control, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China.
Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China.
Sci Rep. 2017 Oct 23;7(1):13778. doi: 10.1038/s41598-017-14137-x.
The striped stem borer, Chilo suppressalis Walker (Lepidoptera: Crambidae), is one of the most serious rice pests. Besides attacking rice, it also feeds on an economically important vegetable crop, water-oat Zizania latifolia. The species feeding on water-oat has higher growth and survival rate than those on rice, suggesting their success in adaptation to the new host plant. However, little is known about the molecular mechanisms of host plant adaptation. Here we investigated the midgut transcriptome responses of C. suppressalis larvae reared on rice and water-oat. A total of 1,633 differentially expressed genes were identified, with a greater number up-regulated on the more delicious new host. The up-regulation of most digestive and detoxification-related genes may be the result of adaptation to the changes in nutritional requirements and toxic chemicals during host shift. In contrast, down-regulation of ribosomal genes may be related to their better development performance when feeding on the new host. In conclusion, our results suggest that transcriptional regulation of genes related to digestion, detoxification and ribosome may play an important role in adaptation of C. suppressalis to a new host plant.
条纹稻苞虫，Chilo suppressalis Walker（鳞翅目：草螟科），是水稻上最严重的害虫之一。它除了取食水稻外，还取食一种重要的经济蔬菜作物菰。取食菰的幼虫比取食水稻的幼虫具有更高的生长和存活率，表明它们成功地适应了新的寄主植物。然而，对于寄主植物适应的分子机制知之甚少。在这里，我们研究了饲养在水稻和菰上的条纹稻苞虫幼虫的中肠转录组应答。共鉴定到 1633 个差异表达基因，其中更多的基因在更美味的新宿主上上调。大多数与消化和解毒相关的基因的上调可能是由于在寄主转移过程中对营养需求和有毒化学物质的变化的适应。相比之下，核糖体基因的下调可能与它们在新寄主上更好的发育表现有关。总之，我们的研究结果表明，与消化、解毒和核糖体相关的基因的转录调控可能在条纹稻苞虫适应新的寄主植物方面发挥了重要作用。
